MBP Selector

Details zu Produkt Nr. ABIN5311503, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Escherichia coli (E. coli)
Wirt
Camelid (Camelidae)
Applikation
Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Proben Cell Extracts
Spezifität The Antibody with Clone 1G5 recognizes E.coli maltose-binding protein (MBP)
Produktmerkmale MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. MBP Selector thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background. MBP Selector is compatible not only with physiological buffers but also with high stringency buffers. MBP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Bestandteile 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
Benötigtes Material wash buffers, columns, tubes
Bead Ligand Antibody
Bead Matrix Agarose beads
Bead Size 90 μm
Andere Bezeichnung maltose binding protein, MBP
Hintergrund Maltose-binding protein (MBP) is encoded by the malE gene from the gram-negative bacterium Escherichia coli. When expressed as a fusion protein it boosts the expression of the fusion partner and is therefore a common entity in bacterial expression vectors
Applikationshinweise 200 μL slurry for up to 10 reactions
Kommentare

Capacity: > 2.5 μg MBP per μl of packed beads (i.e. 2 μl of slurry)

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer 50 % slurry in 20 % ethanol / PBS 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Bilder des Herstellers
Immunoprecipitation (IP) image for MBP Selector (ABIN5311503) Immunoprecipitation of MBP (•) from HeLa lysate. In/Ft: 1/1000 of input and non-bound...