Human TNFSF12 ELISA Kit

Recommended TNFSF12 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA Kits
  • APO3L
  • DR3LG
  • TWEAK
  • Apo3l
  • Dr3l
  • Dr3lg
  • Tweak
  • tumor necrosis factor (ligand) superfamily, member 12
  • tumor necrosis factor ligand superfamily member 12
  • TNFSF12
  • Tnfsf12
Epitop
AA 94-249
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
17
10
9
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN1672836
$ 369.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN419579 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Anmelden zum Anzeigen
10.090268 ABIN924803 Sandwich ELISA Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2703545 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 40-3.000 pg/mL 40 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2708405 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN1572811 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN3032998 Competition ELISA Cell Culture Supernatant, Plasma, Serum 46.9-3000 pg/mL 46.9 pg/mL Anmelden zum Anzeigen
1 ABIN5594712 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN5659651 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.62 pg/mL - 1000 pg/mL 15.62 pg/mL Anmelden zum Anzeigen
1 ABIN456722 Sandwich ELISA Serum, Tissue Homogenate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN5648414 Sandwich ELISA Biological Samples, Cell Culture Supernatant, Cell Lysate, Serum 8 pg/mL - 500 pg/mL 8 pg/mL Anmelden zum Anzeigen
1 ABIN5524728 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
1 ABIN5659687 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 46.9 pg/mL - 3000 pg/mL 46.9 pg/mL Anmelden zum Anzeigen
1 ABIN2115099 Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Anmelden zum Anzeigen
1 ABIN2042702 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2685742 Sandwich ELISA Cell Lysate, Plasma, Serum Anmelden zum Anzeigen
1 ABIN2540104 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Anmelden zum Anzeigen
1 ABIN4973407 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA Kits
Epitop AA 94-249
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(17), (10), (9)
Methodentyp Sandwich ELISA
Detektionsbereich 62.5-4000 pg/mL
Untere Nachweisgrenze 62.5 pg/mL
Applikation ELISA
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails TNFSF12 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für TNFSF12 Kit (ABIN1672836) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF12
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli
Immunogen sequence: S94-H249
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S94-H249

Antigendetails

Produktdetails TNFSF12 ELISA Kit Anwendungsinformationen Handhabung Referenzen für TNFSF12 Kit (ABIN1672836) Bilder zurück nach oben
Antigen
Andere Bezeichnung TNFSF12 (TNFSF12 ELISA Kit Abstract)
Hintergrund

Protein Function: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. .

Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis(TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.

Synonyms: Tumor necrosis factor ligand superfamily member 12,APO3 ligand,TNF-related weak inducer of apoptosis,TWEAK,Tumor necrosis factor ligand superfamily member 12, membrane form,Tumor necrosis factor ligand superfamily member 12, secreted form,TNFSF12,APO3L, DR3LG,UNQ181/PRO207,

Full Gene Name: Tumor necrosis factor ligand superfamily member 12

Cellular Localisation: Cell membrane, Single-pass type II membrane protein.
Gen-ID 8742
UniProt O43508
Pathways Apoptose

Anwendungsinformationen

Produktdetails TNFSF12 ELISA Kit Antigendetails Handhabung Referenzen für TNFSF12 Kit (ABIN1672836) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the tumor necrosis factor family.

Tissue Specificity: Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.

Plattentyp Pre-coated
Protokoll human TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF12 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFSF12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFSF12 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 588, Standard deviation: 26.46, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 1202, Standard deviation: 65, CV(%): 5.4
  • Sample 3: n=16, Mean(pg/ml): 2235, Standard deviation: 84.93, CV(%): 3.8,
  • Sample 1: n=24, Mean(pg/ml): 634, Standard deviation: 37.41, CV(%): 5.9
  • Sample 2: n=24, Mean(pg/ml): 1127, Standard deviation: 72.13, CV(%): 6.4
  • Sample 3: n=24, Mean(pg/ml): 2446, Standard deviation: 115, CV(%): 4.7
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails TNFSF12 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für TNFSF12 Kit (ABIN1672836) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen für TNFSF12 Kit (ABIN1672836)

Produktdetails TNFSF12 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Chicheportiche, Bourdon, Xu, Hsu, Scott, Hession, Garcia, Browning: "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32401-10, 1998

Bilder

Produktdetails TNFSF12 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für TNFSF12 Kit (ABIN1672836) zurück nach oben
Bilder des Herstellers
ELISA image for Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA Kit (ABIN1672836) Human TNFSF12 PicoKine ELISA Kit standard curve