anti-Human TNFSF10 Antikörper für Biochemical Assay

Recommended TNFSF10 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) Antikörper
  • TRAIL
  • tl2
  • apo2l
  • cd253
  • trail
  • apo-2l
  • trail1
  • xtrail1
  • APO2L
  • Apo-2L
  • CD253
  • TL2
  • A330042I21Rik
  • AI448571
  • APO-2L
  • Ly81
  • Trail
  • tumor necrosis factor (ligand) superfamily, member 10
  • TNF-related apoptosis inducing ligand-like
  • TNFSF10
  • TRAIL-LIKE
  • tnfsf10
  • Tnfsf10
Reaktivität
Human
321
74
34
3
3
2
1
1
1
Wirt
Maus
191
152
24
2
1
Klonalität
Monoklonal
Konjugat
Dieser TNFSF10 Antikörper ist unkonjugiert
38
21
14
10
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Biochemical Assay (BCA), Blocking Reagent (BR), Flow Cytometry (FACS)
239
117
113
53
49
28
17
14
13
13
10
8
4
2
2
2
2
1
1
1
1
1
Optionen
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Produktnummer ABIN2689129
192,50 €
Zzgl. Versandkosten 20,00 € und MWSt

anti-TNFSF10 Antikörper für Biochemical Assay mit den meisten Publikationen

Ähnliche anti-TNFSF10 Antikörper

Applikation / Reaktivität Human
Biochemical Assay (BCA) 1 Antikörper
Blocking Antibody (Inhibition) 2 Antikörper
Blocking Reagent (BR) 2 Antikörper
Cell Culture (CC) 1 Antikörper
ELISA 101 Antikörper
ELISA (Capture) 2 Antikörper
ELISA (Detection) 2 Antikörper
ELISpot (Capture) (ELISPOT (Capture)) 1 Antikörper
ELISpot (Detection) (ELISPOT (Detection)) 1 Antikörper
Enzyme Immunoassay (EIA) 8 Antikörper
Flow Cytometry (FACS) 91 Antikörper
Functional Studies (Func) 24 Antikörper
Immunochromatography (IC) 2 Antikörper
Immunocytochemistry (ICC) 4 Antikörper
Immunofluorescence (IF) 13 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 1 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antikörper
Immunohistochemistry (IHC) 45 Antikörper

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) Antikörper
Reaktivität Human
(321), (74), (34), (3), (3), (2), (1), (1), (1)
Wirt Maus
(191), (152), (24), (2), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TNFSF10 Antikörper ist unkonjugiert
(38), (21), (14), (10), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Biochemical Assay (BCA), Blocking Reagent (BR), Flow Cytometry (FACS)
(239), (117), (113), (53), (49), (28), (17), (14), (13), (13), (10), (8), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TNFSF10 Antikörper

Target Details TNFSF10 Anwendungsinformationen Handhabung ProductDetails: References for anti-TNFSF10 antibody (ABIN2689129) Bilder
Marke BD Pharmingen™
Produktmerkmale The RIK-2 monoclonal antibody specifically binds to CD253 which is also known as TRAIL (TNF-Related Apoptosis-Inducing Ligand) and Apo2 ligand (APO-2L). CD253 is a member of the Tumor Necrosis Factor Superfamily and is encoded by the TNFSF10 gene. CD253 is a type II membrane protein that may be expressed as a full-length, cell surface associated protein as well as in a soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis by a wide range of cell lines but not by normal cells. TRAIL is expressed by activated T cells, NK cells, dendritic cells, monocytes and by a variety of non-lymphoid cells. TRAIL can bind to and exert apoptosis through DR4 (TRAIL-R1) and DR5 (TRAIL-R2) receptors. It can also bind to decoy receptors, including DcR1/TRID/TRAIL-R3 and DcR2/TRUNDD/TRAIL-R4, and possibly OPG/TNFRSF11B, which may serve to regulate TRAIL activity. TRAIL has been shown to be involved in T cell-mediated cytotoxicity, however its mechanism of action remains to be fully elucidated. The RIK-2 clone was selected based on its ability to block TRAIL-mediated cytotoxic activity. Flow Cytometric Analysis of TRAIL-induced Killing and RIK-2 Blocking using FITC Annexin V staining. Jurkat T cells were left untreated (far left/first panel) or treated for 16 hours under the following conditions: Cells were incubated with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody (second panel), or with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody preincubated with RIK-2 antibody (third panel), or with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody preincubated with mIgG1 antibody (negative control) (far right/fourth panel). Following treatments, cells were evaluated for Annexin-V staining. Cells induced to undergo apoptosis by treatment with recombinant human TRAIL gave a population of cells that was Annexin V-FITC positive (second panel, M2). Annexin V-FITC staining was blocked when cells were incubated with a mixture of recombinant human TRAIL and RIK-2 antibody (third panel, M2). Cells treated with the mixture of recombinant TRAIL and mIgG1 antibody could not block the killing (far right/fourth panel). A small number of Annexin-V positive cells in the untreated population represents a basal level of apoptosis (far left/first panel). The results indicate that clone RIK-2 can block cell mediated killing induced by recombinant human TRAIL as measured by Annexin V-FITC staining of Jurkat cells.

BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD253 - NA/LE - Clone RIK-2 - Isotype Mouse IgG1 - Reactivity Hu - 0.25 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterilität Sterile filtered,0.2 μm filtered
Endotoxin-Niveau Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Human TRAIL Transfected Cell Line
Klon RIK
Isotyp IgG1

Target Details TNFSF10

Produktdetails anti-TNFSF10 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-TNFSF10 antibody (ABIN2689129) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD253 (TNFSF10 Antibody Abstract)
Hintergrund Synonyms: TNFSF10, TRAIL, APO-2L, TL2
Pathways Apoptose, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-TNFSF10 Antikörper Target Details TNFSF10 Handhabung ProductDetails: References for anti-TNFSF10 antibody (ABIN2689129) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TNFSF10 Antikörper Target Details TNFSF10 Anwendungsinformationen ProductDetails: References for anti-TNFSF10 antibody (ABIN2689129) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Konservierungsmittel Without preservative
Handhabung This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

ProductDetails: References for anti-TNFSF10 antibody (ABIN2689129)

Produktdetails anti-TNFSF10 Antikörper Target Details TNFSF10 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Kayagaki, Yamaguchi, Nakayama, Kawasaki, Akiba, Okumura, Yagita: "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2639-47, 1999

Marsters, Pitti, Donahue, Ruppert, Bauer, Ashkenazi: "Activation of apoptosis by Apo-2 ligand is independent of FADD but blocked by CrmA." in: Current biology : CB, Vol. 6, Issue 6, pp. 750-2, 1997

Sheridan, Marsters, Pitti, Gurney, Skubatch, Baldwin, Ramakrishnan, Gray, Baker, Wood, Goddard, Godowski, Ashkenazi: "Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors." in: Science (New York, N.Y.), Vol. 277, Issue 5327, pp. 818-21, 1997

Pitti, Marsters, Ruppert, Donahue, Moore, Ashkenazi: "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family." in: The Journal of biological chemistry, Vol. 271, Issue 22, pp. 12687-90, 1996

Wiley, Schooley, Smolak, Din, Huang, Nicholl, Sutherland, Smith, Rauch, Smith: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, 1996

Bilder

Produktdetails anti-TNFSF10 Antikörper Target Details TNFSF10 Anwendungsinformationen Handhabung ProductDetails: References for anti-TNFSF10 antibody (ABIN2689129) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-TNFSF10 Antikörper (Tumor Necrosis Factor (Ligand) Superfamily, Member 10) (ABIN2689129) anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) antibody