anti-Maus FASL Antikörper für Immunohistochemistry (Frozen Sections)

Recommended FASL Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand L homeolog
  • Fas cell surface death receptor
  • Fas ligand
  • Fas ligand (TNF superfamily, member 6)
  • faslg.L
  • FAS
  • FASLG
  • faslg
  • fas
  • Fasl
  • Faslg
Epitop
AA 196-220
26
19
17
16
12
9
8
7
7
6
5
5
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Maus
421
173
89
36
7
5
5
3
3
2
1
1
Wirt
Ratte
259
176
49
32
28
7
1
Klonalität
Monoklonal
Konjugat
Dieser FASL Antikörper ist unkonjugiert
45
31
27
13
12
12
9
9
9
8
8
8
8
8
7
6
6
3
2
2
2
2
2
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
313
257
124
95
79
75
51
47
28
27
13
11
10
10
7
5
3
2
2
1
1
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN4310543
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN187288 IHC (fro) IHC (p) Rat IgM Anmelden zum Anzeigen A11

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
Epitop AA 196-220
(26), (19), (17), (16), (12), (9), (8), (7), (7), (6), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Maus
(421), (173), (89), (36), (7), (5), (5), (3), (3), (2), (1), (1)
Wirt Ratte
(259), (176), (49), (32), (28), (7), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser FASL Antikörper ist unkonjugiert
(45), (31), (27), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (7), (6), (6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Applikation Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(313), (257), (124), (95), (79), (75), (51), (47), (28), (27), (13), (11), (10), (10), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FASL Antikörper

Target Details FASL Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN4310543) Bilder
Reinigung Protein G purified
Immunogen Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L, APO-1L, CD178).
Klon A11
Isotyp IgM

Target Details FASL

Produktdetails anti-FASL Antikörper Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN4310543) Bilder zurück nach oben
Antigen
Andere Bezeichnung Fas Ligand/TNFSF6 (FASL Antibody Abstract)
Hintergrund Gene Symbol: FASLG
Gen-ID 356
Pathways Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-FASL Antikörper Target Details FASL Handhabung Referenzen für anti-FASL Antikörper (ABIN4310543) Bilder zurück nach oben
Applikationshinweise Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody. Method: Cryostat sections (6-8um) were air dried, fixed in 4 % paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 μg/mL of A11 antibody in PBS, 0.2 % BSA, 0.05 % Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Paraffin sections: Dewax in xylene (2x10 min), EtOH 100 % (2x10 min), block endogeneous peroxidase (0.6 % H2O2 in Methanol 10 min), rehydrate through graded EtOH (100 %, 95 %, 70 %), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 μg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP (ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC).
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Referenzen für anti-FASL Antikörper (ABIN4310543) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.

Referenzen für anti-FASL Antikörper (ABIN4310543)

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Viard-Leveugle, Bullani, Meda, Micheau, Limat, Saurat, Tschopp, French: "Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16183-8, 2003

Sata, Luo, Walsh: "Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 11, pp. 6964-71, 2001

Refaeli, Van Parijs, London, Tschopp, Abbas: "Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis." in: Immunity, Vol. 8, Issue 5, pp. 615-23, 1998

Li, Rosen, Ronen, Behrens, Krammer, Clark, Berke: "The regulation of CD95 ligand expression and function in CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 8, pp. 3943-9, 1998

Saas, Walker, Hahne, Quiquerez, Schnuriger, Perrin, French, Van Meir, de Tribolet, Tschopp, Dietrich: "Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?" in: The Journal of clinical investigation, Vol. 99, Issue 6, pp. 1173-8, 1997

Hahne, Peitsch, Irmler, Schröter, Lowin, Rousseau, Bron, Renno, French, Tschopp: "Characterization of the non-functional Fas ligand of gld mice." in: International immunology, Vol. 7, Issue 9, pp. 1381-6, 1996

French, Hahne, Viard, Radlgruber, Zanone, Becker, Müller, Tschopp: "Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover." in: The Journal of cell biology, Vol. 133, Issue 2, pp. 335-43, 1996

Renno, Hahne, Tschopp, MacDonald: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, 1996

Hahne, Rimoldi, Schröter, Romero, Schreier, French, Schneider, Bornand, Fontana, Lienard, Cerottini, Tschopp: "Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape." in: Science (New York, N.Y.), Vol. 274, Issue 5291, pp. 1363-6, 1996

Knowles, Moncada: "Nitric oxide synthases in mammals." in: The Biochemical journal, Vol. 298 ( Pt 2), pp. 249-58, 1994

Bilder

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN4310543) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 196-220) antibody (ABIN4310543) Immunohistochemistry-Paraffin: Fas Ligand Antibody (A11) [NBP1-97519] - Immunohistoch...