anti-Ratte (Rattus) Caspase 8 Antikörper für Immunofluorescence (Cultured Cells)

Recommended Caspase 8 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antikörper
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8, apoptosis-related cysteine peptidase
  • death related ced-3/Nedd2-like protein
  • caspase-8
  • caspase 8
  • casp8
  • Dredd
  • CASP8
  • AaeL_AAEL014148
  • LOC100222284
  • Casp8
  • LOC100348477
Epitop
AA 430-482
44
19
18
16
14
10
10
9
9
8
7
6
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
353
117
94
25
21
20
3
2
1
Wirt
Kaninchen
270
114
10
2
Klonalität
Polyklonal
Konjugat
Dieser Caspase 8 Antikörper ist unkonjugiert
18
13
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
352
109
89
77
50
36
35
27
13
12
9
8
6
5
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN724205
315,94 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2808101 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN886653 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN724208 IF (cc) IF (p) Cy3 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN724209 IF (cc) IF (p) Cy5 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN724210 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN724211 IF (cc) IF (p) Cy7 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN724212 IF (cc) IF (p) FITC Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN886652 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN886655 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN886654 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN4999086 IF (cc) IF (p) Alexa Fluor 680 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal
1 ABIN4999087 IF (cc) IF (p) Alexa Fluor 750 Rabbit IgG AA 430-482 Anmelden zum Anzeigen Polyclonal

anti-Caspase 8 Antikörper für Immunofluorescence (Cultured Cells) mit den meisten Publikationen

Ähnliche anti-Caspase 8 Antikörper

Applikation / Reaktivität Ratte (Rattus) Maus
ELISA 18 Antikörper 32 Antikörper
Enzyme Immunoassay (EIA) 2 Antikörper 4 Antikörper
Flow Cytometry (FACS) 7 Antikörper 12 Antikörper
Immunocytochemistry (ICC) 11 Antikörper 15 Antikörper
Immunofluorescence (IF) 7 Antikörper 7 Antikörper
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antikörper 13 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antikörper 13 Antikörper
Immunohistochemistry (IHC) 32 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antikörper
Immunoprecipitation (IP) 11 Antikörper
Western Blotting (WB) 76 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 2 Antikörper

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antikörper
Epitop AA 430-482
(44), (19), (18), (16), (14), (10), (10), (9), (9), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(353), (117), (94), (25), (21), (20), (3), (2), (1)
Wirt Kaninchen
(270), (114), (10), (2)
Klonalität
Polyklonal
Konjugat Dieser Caspase 8 Antikörper ist unkonjugiert
(18), (13), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(352), (109), (89), (77), (50), (36), (35), (27), (13), (12), (9), (8), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 8 Antikörper

Target Details Caspase 8 Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 8 antibody (ABIN724205) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10
Isotyp IgG

Target Details Caspase 8

Produktdetails anti-Caspase 8 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 8 antibody (ABIN724205) Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase 8 (CASP8 Antibody Abstract)
Hintergrund

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

Subcellular location: Cytoplasm

Synonyms: MACH, Mch5, FLICE, CASP-8, Caspase-8, Casp8

Gen-ID 12370
UniProt O89110
Forschungsgebiet Proteolysis / Ubiquitin, Apoptosis/Necrosis
Pathways Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Handhabung ProductDetails: References for anti-Caspase 8 antibody (ABIN724205) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Anwendungsinformationen ProductDetails: References for anti-Caspase 8 antibody (ABIN724205) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

ProductDetails: References for anti-Caspase 8 antibody (ABIN724205)

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Tulsulkar, Glueck, Hinds, Shah: "Ginkgo biloba Extract Prevents Female Mice from Ischemic Brain Damage and the Mechanism Is Independent of the HO1/Wnt Pathway." in: Translational stroke research, Vol. 7, Issue 2, pp. 120-31, 2016

Akinrinde, Oyagbemi, Omobowale, Asenuga, Ajibade: "Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 36, pp. 27-37, 2016 (Probematerial (Species): Rat (Rattus)).

Gao, Liu, Li, Wu, Peng, Jing: "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer." in: American journal of translational research, Vol. 8, Issue 2, pp. 1115-32, 2016

Daverey, Agrawal: "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." in: Neuroscience, Vol. 333, pp. 92-103, 2016

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, 2016

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Pig (Porcine)).

Fang, Zhang, Qi, Fan, Luo, Liu, Tan: "Evodiamine induces G2/M arrest and apoptosis via mitochondrial and endoplasmic reticulum pathways in H446 and H1688 human small-cell lung cancer cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e115204, 2014

Wang, Han, Zhou, Wu, Wang, Jia, Lv, Cheng, Pan, Liu, Zhou, Sun: "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 1, pp. 28-42, 2013

Wu, Tang, Jiang, Li, Jiang, Liu: "PCSK9 siRNA inhibits HUVEC apoptosis induced by ox-LDL via Bcl/Bax-caspase9-caspase3 pathway." in: Molecular and cellular biochemistry, Vol. 359, Issue 1-2, pp. 347-58, 2011

Jeng, Dyson: "Direct measurement of the aspartic acid 26 pKa for reduced Escherichia coli thioredoxin by 13C NMR." in: Biochemistry, Vol. 35, Issue 1, pp. 1-6, 1996

Bilder

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 8 antibody (ABIN724205) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 430-482) (ABIN724205) Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase...
SDS-PAGE (SDS) image for anti-Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 430-482) (ABIN724205) L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal ...
Western Blotting (WB) image for anti-Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 430-482) (ABIN724205) Image provided by the Independent Validation Program (badge number 29759). Lane 1: He...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 430-482) (ABIN724205) Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit An...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 430-482) (ABIN724205) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
'Independent Validation' Siegel
Chargennummer 131127
Validierte Anwendung Western Blotting
Positivkontrolle HeLa cells
Negativkontrolle c6/36 mosquito cells (non-reactive species)
Bewertung A strong band was observed at the correct molecular weight in the positive control sample. No major bands were observed in the negative sample.
Primärantikörper
Sekundärantikörper
Kontrollen
  • Positive control: HeLa cell extract
  • Negative control: c6/36 cell extract
Protokoll
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (CASP8; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Anmerkungen - Nothing to note.
Validierungsbilder
Western Blotting validation image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Figure 1: Western blot of lysates from HeLa cells (Lane 1), and c6/36 cells (Lane 2) ...