anti-Maus Caspase 3 Antikörper für Western Blotting

Recommended Caspase 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Reaktivität
Human, Maus, Ratte (Rattus)
566
304
253
48
47
38
29
28
24
20
18
18
18
12
9
8
7
4
2
2
Wirt
Maus
458
161
19
4
2
Klonalität
Monoklonal
Konjugat
Dieser Caspase 3 Antikörper ist unkonjugiert
27
24
19
14
9
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
482
237
219
147
95
78
55
51
44
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN3188045
154,33 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.3471746 ABIN222873 IHC WB Rabbit IgG Cleavage Site, N-Term, active Anmelden zum Anzeigen Polyclonal 24
3.3471746 ABIN400729 IHC (p) WB Mouse IgG Anmelden zum Anzeigen 31A1067 1
3.3471746 ABIN1536103 ELISA WB Rabbit IgG Cleaved-Asp175 Anmelden zum Anzeigen Polyclonal 1
3.3471746 ABIN222951 WB Rabbit IgG C-Term, Large Subunit Anmelden zum Anzeigen Polyclonal 8
3.3471746 ABIN724625 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-100 Anmelden zum Anzeigen Polyclonal 62
3.3471746 ABIN153016 IHC IHC (p) SimWes WB Mouse IgG2a kappa Anmelden zum Anzeigen CPP32 4-1-18 18
3.3471746 ABIN252651 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Anmelden zum Anzeigen 31A1067 107
3.3471746 ABIN222869 WB Mouse IgG1 Anmelden zum Anzeigen C33 7
3.3471746 ABIN968341 IF IP WB Mouse IgG1 AA 25-145 Anmelden zum Anzeigen 46-Caspase 5
3.3471746 ABIN968342 IF IP WB Mouse IgG1 AA 25-145 Anmelden zum Anzeigen 46-Caspase 5
3.3471746 ABIN2668594 WB Mouse IgG1 Anmelden zum Anzeigen 31A1067 2
3.3471746 ABIN2787903 IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN3043802 IHC (p) WB Rabbit IgG AA 67-175 Anmelden zum Anzeigen Polyclonal 68
3.3471746 ABIN4288099 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
3.3471746 ABIN3023067 IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
3.3471746 ABIN126918 WB Mouse IgG1 active Anmelden zum Anzeigen C33
3.3471746 ABIN1105675 WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN1531535 IHC ELISA WB Rabbit IgG pSer150 Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN1536080 IHC ELISA WB Rabbit IgG Cleaved-Asp175 Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN2470477 IHC WB Mouse IgG full length Anmelden zum Anzeigen 31A1067

anti-Caspase 3 Antikörper für Western Blotting mit den meisten Publikationen

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Applikation / Reaktivität Human Maus Ratte (Rattus)
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Western Blotting (WB) 447 Antikörper 214 Antikörper 206 Antikörper
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Immunostaining (ISt) 1 Antikörper
Immunoprecipitation (IP) 72 Antikörper 35 Antikörper 31 Antikörper

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
Reaktivität Human, Maus, Ratte (Rattus)
(566), (304), (253), (48), (47), (38), (29), (28), (24), (20), (18), (18), (18), (12), (9), (8), (7), (4), (2), (2)
Wirt Maus
(458), (161), (19), (4), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Caspase 3 Antikörper ist unkonjugiert
(27), (24), (19), (14), (9), (9), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunohistochemistry (IHC), Western Blotting (WB)
(482), (237), (219), (147), (95), (78), (55), (51), (44), (29), (28), (13), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 3 Antikörper

Target Details Caspase 3 Anwendungsinformationen Handhabung Bilder
Spezifität The antibody detects endogenous Active Caspase-3 protein.
Produktmerkmale Mouse Monoclonal to Active Caspase-3.
Reinigung The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Immunogen Recombinant Protein
Klon 5E1
Isotyp IgG1

Target Details Caspase 3

Produktdetails anti-Caspase 3 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase-3 (CASP3 Antibody Abstract)
Gen-ID 836
UniProt P42574
Forschungsgebiet Autophagy, Apoptosis/Necrosis
Pathways Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Handhabung Bilder zurück nach oben
Applikationshinweise WB 1:500-1000, IHC 1:100-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze/thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C.

Bilder

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Caspase 3 Antikörper (Caspase 3, Apoptosis-Related Cysteine Peptidase) (ABIN3188045) anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody
Immunohistochemistry (IHC) image for anti-Caspase 3 Antikörper (Caspase 3, Apoptosis-Related Cysteine Peptidase) (ABIN3188045) Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil Tissue using Ac...
'Independent Validation' Siegel
Antigen Caspase 3 (active)
Validierte Anwendung Western Blotting
Positivkontrolle Mouse cerebellar tissue samples at post natal day P5
Bewertung The antibody recognized both the active and non-active form of Caspase3 and worked well in the experimental conditions tested. The product is therefore indicated for the WB use.
Sekundärantikörper ABIN3179097
Protokoll
  • Mouse cerebellar tissue samples at post natal day (P) 5 were lysed in 150 µL of lysis buffer.
  • Protein concentration was determined using Bradford reagen (Sigma B6916) according to the manufacturer's recommendation.
  • 30 and 60µg total protein were denatured in sample buffer for 5min at 95°C and then cooled on ice.
  • Proteins were separated on a denaturing homemade 15% SDS-PAGE gel at a constant voltage of 180V for 70min.
  • Proteins were transferred in a transfer buffer to nitrocellulose membrane (Amersham Biosciences, RPN203D, lot 18100/66/0207) at constant amperage of 400mA for 60min.
  • Blocking of the membrane with blocking solution (0.01M PBS, 0.1% tween, 2% Bovine Serum Albumin) for 1h at RT.
  • Incubation with the primary antibody ABIN3179097 diluted 1:1000 in blocking solution ON at 4°C.
  • 3 washes in PBS-0.1% tween for 15min at RT.
  • Incubation with the anti-mouse horseradish peroxidase (HRP) secondary antibody (Santa Cruz, SC2005, lot H0406) diluted 1:10000 in blocking solution for 1h at RT.
  • Washes in PBS-0.1% tween for 15min at RT.
  • Chemiluminescence detection using homemade ECL (1min incubation at RT) and revealed on HYPERFILM EGL (Amersham Biosciences, RPN2013K, lot 04450300115); exposure 30sec.
  • Proteins were revealed using the ECL technique on western blot films for 30 seconds.
Anmerkungen
  • A band around 30-35 kDa and a band around 17kDa were detected with the experimental procedures previously described.
  • A protein load of 30µg is recommended, as in the 60µg load, weak non-specific bands were detected around 15 and 30kDa.
Validierungsbilder
Western Blotting validation image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN3179097) P5 mouse cerebellum, 30 seconds exposition. 60µg (left) and 30µg (right) protein load...