anti-Human Caspase 3 Antikörper für Intracellular Staining

Recommended Caspase 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Reaktivität
Human, Maus
566
304
254
48
47
38
29
28
24
20
18
18
18
12
9
8
7
4
2
2
Wirt
Kaninchen
457
162
19
4
2
Klonalität
Monoklonal
Konjugat
Dieser Caspase 3 Antikörper ist konjugiert mit Biotin
26
24
19
14
9
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Applikation
Intracellular Staining (ICS)
483
238
219
147
95
78
55
51
44
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
Optionen
Hersteller
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Produktnummer ABIN2688869
391,59 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2688868 BI IHC (fro) ICS IP ELISA WB Rabbit IgG Anmelden zum Anzeigen C92 2

anti-Caspase 3 Antikörper für Intracellular Staining mit den meisten Publikationen

Ähnliche anti-Caspase 3 Antikörper

Applikation / Reaktivität Human Maus
Immunofluorescence (fixed cells) (IF/ICC) 6 Antikörper 4 Antikörper
Cytometry by Time of Flight (CyTOF) 4 Antikörper 3 Antikörper
Simple Western (SimWes) 5 Antikörper 5 Antikörper
Western Blotting (WB) 447 Antikörper 214 Antikörper
Proximity Ligation Assay (PLA) 2 Antikörper
Intracellular Staining (ICS) 2 Antikörper 2 Antikörper
Immunostaining (ISt) 1 Antikörper
Immunoprecipitation (IP) 72 Antikörper 35 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 140 Antikörper 74 Antikörper
Immunohistochemistry (IHC) 223 Antikörper 92 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 27 Antikörper

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
Reaktivität Human, Maus
(566), (304), (254), (48), (47), (38), (29), (28), (24), (20), (18), (18), (18), (12), (9), (8), (7), (4), (2), (2)
Wirt Kaninchen
(457), (162), (19), (4), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Caspase 3 Antikörper ist konjugiert mit Biotin
(26), (24), (19), (14), (9), (9), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Intracellular Staining (ICS)
(483), (238), (219), (147), (95), (78), (55), (51), (44), (29), (28), (13), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 3 Antikörper

Target Details Caspase 3 Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN2688869) Bilder
Marke BD Pharmingen™
Produktmerkmale The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3. Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells (Human T-cell leukemia, ATCC TIB-152) were left untreated (left panel) or treated with 4 μM of camptothecin for 4 hr to induce apoptosis (right panel). Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714) for 20 min at room temperature (RT), pelleted and washed with BD Perm/Wash™ buffer (component of Cat. No. 554714). Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody (clone C92-605). Cells were then washed and resuspended in BD Perm/Wash™ buffer containing FITC-Avidin (5 ng/1x10e6 cells) [Cat. No. 554057] and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash™ buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 (left panel, M1), whereas more than half of the treated cells were positive for active caspase-3 staining (right panel, M2).

BD Pharmingen™ Biotin Rabbit Anti- Active Caspase-3 - Biotin - Clone C92-605 - Isotype Rabbit IgG - Reactivity Hu, Ms - 100 Tests
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Klon C92
Isotyp IgG

Target Details Caspase 3

Produktdetails anti-Caspase 3 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN2688869) Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase-3 (CASP3 Antibody Abstract)
Hintergrund Synonyms: CPP32, Yama, Apopain
Forschungsgebiet Apoptosis/Necrosis, Autophagy
Pathways Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Handhabung ProductDetails: References for anti-Caspase 3 antibody (ABIN2688869) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen ProductDetails: References for anti-Caspase 3 antibody (ABIN2688869) Bilder zurück nach oben
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

ProductDetails: References for anti-Caspase 3 antibody (ABIN2688869)

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Dai, Krantz: "Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." in: Blood, Vol. 93, Issue 10, pp. 3309-16, 1999

Fujita, Tsuruo: "Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis." in: Biochemical and biophysical research communications, Vol. 246, Issue 2, pp. 484-8, 1998

Thornberry, Lazebnik: "Caspases: enemies within." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, 1998