anti-Human Caspase 3 Antikörper für Intracellular Staining

Recommended Caspase 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Reaktivität
Human, Maus
578
308
255
47
46
37
29
27
24
20
18
17
17
12
9
8
7
4
2
2
Wirt
Kaninchen
468
163
19
4
2
Klonalität
Monoklonal
Konjugat
Dieser Caspase 3 Antikörper ist unkonjugiert
27
24
19
14
10
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Applikation
BioImaging (BI), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
491
241
220
146
96
75
55
52
46
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
1
1
1
1
Optionen
Hersteller
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Produktnummer ABIN2688868
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2688869 ICS Biotin Rabbit IgG Anmelden zum Anzeigen C92 3

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
Reaktivität Human, Maus
(578), (308), (255), (47), (46), (37), (29), (27), (24), (20), (18), (17), (17), (12), (9), (8), (7), (4), (2), (2)
Wirt Kaninchen
(468), (163), (19), (4), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Caspase 3 Antikörper ist unkonjugiert
(27), (24), (19), (14), (10), (9), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation BioImaging (BI), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(491), (241), (220), (146), (96), (75), (55), (52), (46), (29), (28), (13), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 3 Antikörper

Target Details Caspase 3 Anwendungsinformationen Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN2688868) Bilder
Marke BD Pharmingen™
Produktmerkmale The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3. Immunoprecipitation/Western blot analysis of caspase-3 from apoptotic and non-apoptotic cell lysates. Jurkat cells (Human T-cell leukemia, ATCC TIB-152) were left untreated (left panel) or were treated with 6 μM camptothecin for 5 hr to induce apoptosis (right panel). Immunoprecipitation: Cell lysates were immunoprecipitated with 0.25 - 2 μg/mL of the rabbit anti- active caspase-3 antibody [clone C92-605, lanes 1 and 8 (2 μg), lanes 2 and 9, (1 μg), lanes 3 and 10 (0.5 μg), and lanes 4 and 11 (0.25 μg)], 1 - 2 μg/mL of a mAb recognizing both pro and active caspase-3 (Cat. No. 610322), lanes 5 and 12 (2 μg), lanes 6 and 13 (1 μg)] or 1 μg/mL of a rabbit IgG isotype control [Jackson Immunoresearch (Cat. No. 011-00000-3), lanes 7 and 14]. Western blot: Caspase-3 was detected by western blot analysis with an antibody that recognizes both pro- (32 kD) and active (20 and 17 kD, reflecting the presence or absence of the caspase-3 pro-domain) caspase-3 [lanes 1-14]. The results also show that the rabbit anti- active caspase-3 antibody (clone C92-605) immunoprecipitated only the active form of caspase-3 (lanes 8-11) as compared to the mouse anti-human caspase-3 antibody (Cat. No. 610322) which immunoprecipitated both the pro and active forms of caspase-3 (lanes 12 and 13). Bands may be observable at ~25 kD and ~55-60 kD which represent the light and heavy chains, respectively, of IgG used for the immunoprecipitation.

BD Pharmingen™ Purified Rabbit Anti- Active Caspase-3 - Purified - Clone C92-605 - Isotype Rabbit IgG - Reactivity Hu, Ms - 25 μg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Active Caspase-3 Fragment
Klon C92
Isotyp IgG

Target Details Caspase 3

Produktdetails anti-Caspase 3 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN2688868) Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase-3 (CASP3 Antibody Abstract)
Molekulargewicht 20 kD & 17 kD
Forschungsgebiet Apoptosis/Necrosis, Autophagy
Pathways Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN2688868) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Referenzen für anti-Caspase 3 Antikörper (ABIN2688868) Bilder zurück nach oben
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.

Referenzen für anti-Caspase 3 Antikörper (ABIN2688868)

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Dai, Krantz: "Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." in: Blood, Vol. 93, Issue 10, pp. 3309-16, 1999

Fujita, Tsuruo: "Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis." in: Biochemical and biophysical research communications, Vol. 246, Issue 2, pp. 484-8, 1998