anti-Human Caspase 3 Antikörper für Fluorescence Microscopy

Recommended Caspase 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Epitop
AA 1-219
43
34
27
24
22
21
19
19
16
15
12
12
12
11
10
10
9
7
6
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
578
309
255
47
46
37
29
27
24
20
18
17
17
12
9
8
7
4
2
2
Wirt
Maus
469
162
19
4
2
Klonalität
Monoklonal
Konjugat
Dieser Caspase 3 Antikörper ist unkonjugiert
27
24
19
14
10
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Applikation
Fluorescence Microscopy (FM), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
491
240
221
146
96
75
55
52
46
29
29
13
9
9
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN967902
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN967901 FM IP IHC WB Mouse IgG2a AA 1-219 Anmelden zum Anzeigen 19-Caspase 5

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antikörper
Epitop AA 1-219
(43), (34), (27), (24), (22), (21), (19), (19), (16), (15), (12), (12), (12), (11), (10), (10), (9), (7), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(578), (309), (255), (47), (46), (37), (29), (27), (24), (20), (18), (17), (17), (12), (9), (8), (7), (4), (2), (2)
Wirt Maus
(469), (162), (19), (4), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Caspase 3 Antikörper ist unkonjugiert
(27), (24), (19), (14), (10), (9), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Fluorescence Microscopy (FM), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(491), (240), (221), (146), (96), (75), (55), (52), (46), (29), (29), (13), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 3 Antikörper

Target Details Caspase 3 Anwendungsinformationen Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN967902) Bilder
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human CPP32 aa. 1-219
Klon 19-Caspase
Isotyp IgG2a

Target Details Caspase 3

Produktdetails anti-Caspase 3 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN967902) Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase-3 (CASP3 Antibody Abstract)
Hintergrund Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is a member of the family of cysteine proteases which includes interleukin-1beta converting enzyme (ICE) and C. elegans protein, Ced-3. An apoptotic signal such as granzyme B of cytotoxic T-cells (CTLs) or ICE-like proteases induces the intracellular cleavage of Caspase-3 from the inactive pro-form (32 kDa) to the active form which consists of the p20, p17, and p12 subunits. The active form of Caspase-3 cleaves several other apoptotic proteins including proteins such as DNA Fragmentation Factor (DFF). Apoptosis can be inhibited by coexpression of Bcl-2 as well as inhibitors of Caspase-3 or other members of the family of cysteine proteases. This antibody recognizes the human pro-form (inactive) of Caspase-3 at 32 kDa. In addition, it has been reported to recognize the active form of Caspase-3 at 20-21 kD in apoptotic cell lysates.
Synonyms: CPP32
Molekulargewicht 32 kDa
Forschungsgebiet Apoptosis/Necrosis, Autophagy
Pathways Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN967902) Bilder zurück nach oben
Kommentare

Related Products: ABIN968537, ABIN967389, ABIN967299

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Referenzen für anti-Caspase 3 Antikörper (ABIN967902) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.

Referenzen für anti-Caspase 3 Antikörper (ABIN967902)

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Takemoto, Nagai, Miyawaki, Miura: "Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects." in: The Journal of cell biology, Vol. 160, Issue 2, pp. 235-43, 2003

Li, Chen, Sinogeeva, Gorospe, Wersto, Chrest, Barnes, Liu: "Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49504-10, 2002

Suzuki, Nakabayashi, Takahashi: "Ubiquitin-protein ligase activity of X-linked inhibitor of apoptosis protein promotes proteasomal degradation of caspase-3 and enhances its anti-apoptotic effect in Fas-induced cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 15, pp. 8662-7, 2001

Donoghue, Baden, Lauder, Sobolewski, Pringle: "Immunohistochemical localization of caspase-3 correlates with clinical outcome in B-cell diffuse large-cell lymphoma." in: Cancer research, Vol. 59, Issue 20, pp. 5386-91, 1999

Scaffidi, Fulda, Srinivasan, Friesen, Li, Tomaselli, Debatin, Krammer, Peter: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998

Allgemeine Veröffentlichungen

Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995

Bilder

Produktdetails anti-Caspase 3 Antikörper Target Details Caspase 3 Anwendungsinformationen Handhabung Referenzen für anti-Caspase 3 Antikörper (ABIN967902) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (ABIN967902) Western blot analysis of Caspase-3 on a Jurkat cell lysate (Human T-cell leukemia, AT...
 image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (ABIN967902) anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (Image 2)
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (ABIN967902) anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (Image 3)