Ratte (Rattus) BIRC3 ELISA Kit

Recommended BIRC3 Product (geliefert von: Anmelden zum Anzeigen )

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Baculoviral IAP Repeat Containing 3 (BIRC3) ELISA Kits
  • AIP1
  • API2
  • CIAP2
  • HAIP1
  • HIAP1
  • MALT2
  • MIHC
  • RNF49
  • c-IAP2
  • AW146227
  • Api1
  • Api2
  • Birc3
  • HIAP2
  • IAP1
  • IAP2
  • MIAP1
  • MIAP2
  • MIHB
  • RNF48
  • cIAP1
  • cIAP2
  • mcIAP1
  • rIAP1
  • Birc2
  • cIAP-1
  • cIAP-2
  • AW107670
  • baculoviral IAP repeat-containing 3
  • inhibitor of apoptosis protein-2
  • baculoviral IAP repeat containing 3
  • baculoviral IAP repeat-containing 2
  • BIRC3
  • OpmnVgp074
  • Birc2
  • Birc3
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
2
1
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
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Produktnummer ABIN4885857
719,71 €
Zzgl. Versandkosten 20,00 € und MWSt

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General

Antigen Baculoviral IAP Repeat Containing 3 (BIRC3) ELISA Kits
Reaktivität Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(2), (1)
Methodentyp Sandwich ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails BIRC3 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Custom Rat cIAP-2 ELISA Kit.
Proben Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität The antibody pair provided in this kit recognizes Rat cIAP-2.
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Antigendetails

Produktdetails BIRC3 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung cIAP-2 (BIRC3 ELISA Kit Abstract)
UniProt F7FLN8
Pathways Apoptose, Caspase Kaskade in der Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails BIRC3 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails BIRC3 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months