anti-Human BIRC3 Antikörper für Immunohistochemistry

Recommended BIRC3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Baculoviral IAP Repeat Containing 3 (BIRC3) Antikörper
  • AIP1
  • API2
  • CIAP2
  • HAIP1
  • HIAP1
  • MALT2
  • MIHC
  • RNF49
  • c-IAP2
  • AW146227
  • Api1
  • Api2
  • Birc3
  • HIAP2
  • IAP1
  • IAP2
  • MIAP1
  • MIAP2
  • MIHB
  • RNF48
  • cIAP1
  • cIAP2
  • mcIAP1
  • rIAP1
  • Birc2
  • cIAP-1
  • cIAP-2
  • AW107670
  • baculoviral IAP repeat-containing 3
  • inhibitor of apoptosis protein-2
  • baculoviral IAP repeat containing 3
  • baculoviral IAP repeat-containing 2
  • BIRC3
  • OpmnVgp074
  • Birc2
  • Birc3
Epitop
AA 100-200, Internal Region
31
25
16
11
8
7
6
5
4
4
4
2
2
2
2
2
2
1
Reaktivität
Human
158
44
31
4
Wirt
Kaninchen
105
34
29
Klonalität
Polyklonal
Konjugat
Dieser BIRC3 Antikörper ist unkonjugiert
9
8
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
128
67
59
39
29
16
13
10
5
5
3
1
1
1
1
Hersteller
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Produktnummer ABIN439961
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
9.452184 ABIN4298718 IHC IHC (fro) IHC (p) IP WB Rabbit Anmelden zum Anzeigen Polyclonal 1
9.452184 ABIN252132 IHC (fro) IHC (p) IP IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
9.452184 ABIN3030108 IHC ELISA WB Rabbit Ig Fraction AA 101-131 Anmelden zum Anzeigen Polyclonal
1 ABIN151193 IHC (p) IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 1
1 ABIN222970 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4298725 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 3
1 ABIN5563850 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4965164 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1969997 IHC ELISA FITC Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1961174 IHC ELISA Alkaline Phosphatase (AP) Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1974582 IHC ELISA PE Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1963551 IHC ELISA APC Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1974583 ELISA ICC IF IHC WB PE Rabbit IgG AA 59-88 Anmelden zum Anzeigen Polyclonal
1 ABIN1969998 ELISA ICC IF IHC WB FITC Rabbit IgG AA 59-88 Anmelden zum Anzeigen Polyclonal
1 ABIN1961175 ELISA ICC IF IHC WB Alkaline Phosphatase (AP) Rabbit IgG AA 59-88 Anmelden zum Anzeigen Polyclonal
1 ABIN1963552 ELISA ICC IF IHC WB APC Rabbit IgG AA 59-88 Anmelden zum Anzeigen Polyclonal
1 ABIN2590307 ELISA IF IHC WB Rabbit IgG AA 58-88 Anmelden zum Anzeigen Polyclonal
1 ABIN2590308 ELISA IHC Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4298719 ICC IF IHC IHC (p) WB Biotin Rabbit AA 100-200, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4298727 ICC IF IHC IHC (p) FITC Rabbit AA 100-200, Internal Region Anmelden zum Anzeigen Polyclonal

General

Antigen Baculoviral IAP Repeat Containing 3 (BIRC3) Antikörper
Epitop AA 100-200, Internal Region
(31), (25), (16), (11), (8), (7), (6), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1)
Reaktivität Human
(158), (44), (31), (4)
Wirt Kaninchen
(105), (34), (29)
Klonalität
Polyklonal
Konjugat Dieser BIRC3 Antikörper ist unkonjugiert
(9), (8), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(128), (67), (59), (39), (29), (16), (13), (10), (5), (5), (3), (1), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-BIRC3 Antikörper

Target Details BIRC3 Anwendungsinformationen Handhabung Referenzen für anti-BIRC3 Antikörper (ABIN439961) Bilder
Reinigung Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of human cIAP2 (within residues 100-200). [Swiss-Prot# Q13489]

Target Details BIRC3

Produktdetails anti-BIRC3 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-BIRC3 Antikörper (ABIN439961) Bilder zurück nach oben
Antigen
Andere Bezeichnung cIAP-2/HIAP-1 (BIRC3 Antibody Abstract)
Hintergrund Gene Symbol: BIRC3
Molekulargewicht Theoretical MW: 64 kDa
Gen-ID 330
UniProt Q13489
Pathways Apoptose, Caspase Kaskade in der Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-BIRC3 Antikörper Target Details BIRC3 Handhabung Referenzen für anti-BIRC3 Antikörper (ABIN439961) Bilder zurück nach oben
Applikationshinweise Western Blot 1 μg/mL, Simple Western 1:100, Immunohistochemistry 1:800-1:3200, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:800-1:3200This cIAP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band can be seen at approx. 64 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for cIAP2 Antibody Procedure Guide for NBP1-27972- cIAP2 AntibodyProcedure Guide for NBP1-27972 - cIAP2 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-cIAP2 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.(c) 2009 Novus Biologicals - cIAP2 Antibody - Page 1
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-BIRC3 Antikörper Target Details BIRC3 Anwendungsinformationen Referenzen für anti-BIRC3 Antikörper (ABIN439961) Bilder zurück nach oben
Format Liquid
Konzentration 1.5 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-BIRC3 Antikörper (ABIN439961)

Produktdetails anti-BIRC3 Antikörper Target Details BIRC3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Kurita, Mott, Cazanave, Fingas, Guicciardi, Bronk, Roberts, Fernandez-Zapico, Gores: "Hedgehog inhibition promotes a switch from Type II to Type I cell death receptor signaling in cancer cells." in: PLoS ONE, Vol. 6, Issue 3, pp. e18330, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting

Guicciardi, Mott, Bronk, Kurita, Fingas, Gores: "Cellular inhibitor of apoptosis 1 (cIAP-1) degradation by caspase 8 during TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis." in: Experimental cell research, Vol. 317, Issue 1, pp. 107-16, 2010 (Probematerial (Species): Human). Weitere Details: Western Blotting

Bilder

Produktdetails anti-BIRC3 Antikörper Target Details BIRC3 Anwendungsinformationen Handhabung Referenzen für anti-BIRC3 Antikörper (ABIN439961) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody (ABIN439961) anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody
Western Blotting (WB) image for anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody (ABIN439961) anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody (Image 2)
Simple Western (SimWes) image for anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody (ABIN439961) Simple Western: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Simple Western lane view shows ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody (ABIN439961) Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-27972] - cIAP2 staining i...
Western Blotting (WB) image for anti-Baculoviral IAP Repeat Containing 3 (BIRC3) (AA 100-200), (Internal Region) antibody (ABIN439961) Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HIAD1 staining in Hela cells dete...