Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antikörper

Antigen: Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1)

Synonyme: flt1b, VEGFR1, FLT, Flt-1, sFlt1, VEGFR-1, AI323757, FLT1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN99319

Produktbeschreibung

Weitere Bezeichnung: Flt-1 (Flt-1/VEGFR1)

Immunogen: A synthetic peptide derived from the C-terminus of the Flt-1 precursor of human origin.

Format: Ig-fraction, Purified

Spezifität: This antibody reacts with a 180 kD protein, known as Flt-1/Vascular endothelial growth factor receptor 1. Flt-1 is a cell membrane receptor kinase. Flk-1 has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. This antibody cross reacts with mouse and rat.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Angiosarcoma. Cellular localization: Cytoplasmic Other Applications: Western Blotting

Konzentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human Flt-1 containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Zytokine, Rezeptoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human angiosarcoma stained with Flt-1

Publikationen

Publikationen: Ahmed, Li, Dunk et al.: "Colocalisation of vascular endothelial growth factor and its Flt-1 receptor in human placenta." in: Growth factors (Chur, Switzerland), Vol. 12, Issue 3, pp. 235-43, 1996 (PubMed).

Ankoma-Sey, Matli, Chang et al.: "Coordinated induction of VEGF receptors in mesenchymal cell types during rat hepatic wound healing." in: Oncogene, Vol. 17, Issue 1, pp. 115-21, 1998 (PubMed).