ES Antikörper

Antigen: ES

Klonalität: Monoklonal (NE1-479)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Indirektes ELISA (iELISA)

Produktnummer: ABIN98717

Produktbeschreibung

Immunogen: A peptide corresponding to amino acids 1-12

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: NE1-479

Beschreibung: Endostatin is globular domain found at the C-terminal derived from type XVIII collagen. It serves as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It is an angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). 1 On the surface of endothelial cells, endostatin binds with the integrin alpha(5)beta(1) that activates the Src-kinase pathway. The binding of endostatin with integrins also down-regulates the activity of RhoA GTPase and inhibits signaling pathways mediated by small kinases of the Ras and Raf families. All these events promote disassembly of the actin cytoskeleton, disorders in cell-matrix interactions, and decrease in endotheliocyte mobility, i.e., promote the suppression of angiogenesis. 2 Endostatin is currently being studied as part of cancer research.

Spezifität: Mouse Anti-Human Endostatin (Clone NE1.479) recognizes Human Endostatin and does not appear to cross-react with Mouse Endostatin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Anwendungen

Applikationshinweise: Indirect ELISA, WB. Each Investigator should determine their own optimal working dilution for specific applications.

Konzentration: 0.1 mg/ml

Reinigung: Purified

Buffer: This purified antibody is formulated in 0.025 M phosphate buffered saline (PBS) pH 7.2 containing 50% glycerol.

Lagerung: For long term storage, aliquot in working volumes without diluting and store at 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Forschungsgebiet: Hormone

Beschränkungen: Nur für Forschungszwecke einsetzbar