Cytokeratin 1 (KRT1) Antikörper

Antigen: Cytokeratin 1 (KRT1)

Synonyme: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Klonalität: Monoklonal (Lu-5)

Wirt: Maus

Reaktivität: Human, Rind (Kuh), Ratte (Rattus), Huhn, Schlange, Frosch

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN98198

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin

Immunogen: BALB/C mice were injected with Lung cancer cell line.

Klon: Lu-5

Spezifität: This antibody reacts with cytokeratins of acidic as well as basic subfamilies, i.e. 1,2,3,4,5,6,7,8,10,12,13,14,15,16,17,18 and 19. It reacts with keratinized and corneal epidermis, stratified epithelia, hyperproliferative keratinocytes and simple epithelia. This antibody is a broad spectrum anti pan cytokeratin antibody, which differentiates epithelial from non-epithelial tumors. It cross reacts with cow, rat , chicken, snake and frog.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require predigestion with Pepsin. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Reinigung: Ig Fraction

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human squamous cell carcinoma of lung stained with Cytokeratin Pan antibody

Publikationen

Publikationen: von Overbeck, Staehli, Gudat et al.: "Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5)." in: Virchows Archiv. A, Pathological anatomy and histopathology, Vol. 407, Issue 1, pp. 1-12, 1985 (PubMed).