Keratin 20 (KRT20) Antikörper

Antigen: Keratin 20 (KRT20)

Synonyme: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Klonalität: Monoklonal (Q2)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN98182

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 20

Immunogen: BALB/C mice were immunized with partially purified human keratin 20 expressed in bacterial cells.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: Q2

Spezifität: This antibody with a protein of 46 kD. Cytokeratin 20 is less acidic than other type I cytokeratins and is of interest due to its more restricted expression in tissue. Cytokeratin 20 is expressed primarily in gastric and intestinal epithelium, urothelium and Merkel- cells. Cytokeratin 20 is expressed in adenocarcinomas of colon, stomach, pancreas and the bile system.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Moll, Loewe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).