Cytokeratin 10 (KRT10) Antikörper

Antigen: Cytokeratin 10 (KRT10)

Synonyme: K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik

Klonalität: Monoklonal (LHP1)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN98147

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 10

Immunogen: Crude cytoskeletal extract prepared from the epidermal component of trypsin-split normal adult skin.

Format: Supernatant

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: LHP1

Spezifität: This antibody is specific to human cytokeratin 10. It recognizes a 56.5 kD cytokeratin protein and found in suprabasal layers of keratinizing stratified epithelia, suprabasal layers of non- keratinizing stratified epithelia and in differentiated areas of squamous carcinomas. This antibody is useful in the differentiation of stratified squamous epithelia.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

Leigh, Purkis, Whitehead et al.: "Monospecific monoclonal antibodies to keratin 1 carboxy terminal (synthetic peptide) and to keratin 10 as markers of epidermal differentiation." in: The British journal of dermatology, Vol. 129, Issue 2, pp. 110-9, 1995 (PubMed).