Cytokeratin 5/8 Antikörper

Antigen: Cytokeratin 5/8

Klonalität: Monoklonal (C50)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN98117

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 5 & 8

Immunogen: Cytoskeletal preparation for Hela cells.

Format: Supernatant

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: C50

Spezifität: This antibody is specific to human cytokeratins 5 (58 kD) and 8 (52.5 kD). Cytokeratin 5 is expressed by basal cells and suprabasal cells of stratified epithelium. Cytokeratin 8 is expressed by simple epithelial cells. This antibody can be useful for the detection of wide range of tumors derived from epithelia.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin 5/8 antibod

Publikationen

Publikationen: Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Angus, Purvis, Stock et al.: "NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue." in: The Journal of pathology, Vol. 153, Issue 4, pp. 377-84, 1988 (PubMed).