Keratin 5/6 (KRT5/6) Antikörper

Antigen: Keratin 5/6 (KRT5/6)

Klonalität: Monoklonal (D5-16 B4)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN98116

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 5 & 6

Immunogen: BALB/C mice were injected with cytokeratin 5.

Format: Ig-fraction (prediluted)

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: D5-16 B4

Spezifität: Cytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, the intermediate and the superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight, basic type cytokeratin expressed by proliferating squamous epithelium often paired with cytokeratin 16.

Anwendungen

Applikationshinweise: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Clover, Oates, Edwards: "Anti-cytokeratin 5/6: a positive marker for epithelioid mesothelioma." in: Histopathology, Vol. 31, Issue 2, pp. 140-3, 1997 (PubMed).

Cury, Butcher, Fisher et al.: "Value of the mesothelium-associated antibodies thrombomodulin, cytokeratin 5/6, calretinin, and CD44H in distinguishing epithelioid pleural mesothelioma from adenocarcinoma metastatic to the pleura." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 13, Issue 2, pp. 107-12, 2000 (PubMed).

Otterbach, Buankfalvi, Bergner et al.: "Cytokeratin 5/6 immunohistochemistry assists the differential diagnosis of atypical proliferations of the breast." in: Histopathology, Vol. 37, Issue 3, pp. 232-40, 2000 (PubMed).