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Antikörper
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anti-Cytokeratin 18 (KRT18) Antikörper
Cytokeratin 18 (KRT18) Antikörper
| Antigen |
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| Synonyme |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Klonalität |
Monoklonal (AE3) |
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Wirt
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Reaktivität
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Human (187), Maus (70), Ratte (Rattus) (68), Hund (45), Kaninchen (35), Rind (Kuh) (27), Schwein (23), Huhn (20), Pferd (17), Säugetiere (10), Hamster (6), None (4), Schaf (4), Vogel (2), Katze (2), Affe (2)
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Applikation
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Western Blot (WB) (117), Immunhistochemie (Paraffinschnitte) (IHC (p)) (111), Immunfluoreszenz (IF) (86), Durchflusszytometrie (FACS) (70), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (51), Immunhistochemie (IHC) (34), Immunpräzipitation (IP) (33), Enzyme Immunoassay (EIA) (30), ELISA (24), Immunzytochemie (ICC) (14), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN98102 |
| Menge |
6ml (1 to 4 ug/ml) (Varianten) |
| Preis |
159,62 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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Cytokeratin 1-8
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Immunogen
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BALB/C mice were immunized with human epidermal keratin.
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Format
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Ig-fraction (prediluted)
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Isotyp
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IgG1 kappa (Passende Sekundärantikörper)
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Klon
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AE3
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Spezifität
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This antibody recognizes most of the basic (Type II) keratins. It recognizes keratin polypeptides of 58 and 65 to 67 kD. In keratinized epidermis, 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. This antibody can be used to stain different cell layers in an epithelial tissue and various neoplasms of epithelial origin.
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Applikationshinweise
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic
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Konzentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Forschungsgebiet
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Extrazelluläre Matrix
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Publikationen
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Klein-Szanto, Boysen, Reith: "Keratin and involucrin in preneoplastic and neoplastic lesions. Distribution in the nasal mucosa of nickel workers." in: Archives of pathology & laboratory medicine, Vol. 111, Issue 11, pp. 1057-61, 1987 (PubMed).
Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).
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Alternativen zu Antigen "Cytokeratin 18 (KRT18)", Typ "Antikörper" finden
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Wirte
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Maus (124), Kaninchen (93), Huhn (2), Meerschweinchen (1), Schaf (1)
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Reaktivitäten
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Human (187), Maus (70), Ratte (Rattus) (68), Hund (45), Kaninchen (35), Rind (Kuh) (27), Schwein (23), Huhn (20), Pferd (17), Säugetiere (10), Hamster (6), None (4), Schaf (4), Vogel (2), Katze (2), Affe (2)
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Applikationen
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Western Blot (WB) (117), Immunhistochemie (Paraffinschnitte) (IHC (p)) (111), Immunfluoreszenz (IF) (86), Durchflusszytometrie (FACS) (70), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (51), Immunhistochemie (IHC) (34), Immunpräzipitation (IP) (33), Enzyme Immunoassay (EIA) (30), ELISA (24), Immunzytochemie (ICC) (14), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (1)
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Konjugate
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Biotin (17), FITC (14), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitope
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pSer33 (10), C-Term (5), AA 7-173 (1), Center (1), N-Term (1)
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Alternativen 
