Collagen, Type VI, alpha 1 (COL6A1) Antikörper

Antigen: Collagen, Type VI, alpha 1 (COL6A1)

Synonyme: OPLL, Col6a-1, AI747156, RGD1565398, MGC53502, COL6A1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN97519

Produktbeschreibung

Weitere Bezeichnung: Collagen Type VI

Immunogen: Collagen Type VI from human and bovine placenta

Format: Liquid (sterile filtered)

Beschreibung: Affinity Purified Anti-Collagen Type VI [Rabbit] Minimum Cross Reactivity to Type I, II, III, IV and V Collagens. Background(s): Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. The manufacture extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Spezifität: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Collagen VI at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen VI in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code ABIN101990 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: "Stabilizer: None. Preservative: None Background(s): Antibodies-Online produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet rep

Lagerung: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Loch, Vakhrusheva, Piotrowska et al.: "Different extent of cardiac malfunction and resistance to oxidative stress in heterozygous and homozygous manganese-dependent superoxide dismutase-mutant mice." in: Cardiovascular research, Vol. 82, Issue 3, pp. 448-57, 2009 (PubMed).