Collagen, Type II (COL2) Antikörper

Antigen: Collagen, Type II (COL2)

Synonyme: COL2A1, LOC100009005

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN97489

Produktbeschreibung

Weitere Bezeichnung: Collagen Type II

Immunogen: Collagen Type II from adult human knee cartilage and bovine nasal cartilage.

Format: Liquid (sterile filtered)

Spezifität: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Collagen II at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen II in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code ABIN101990 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Konzentration: 0.7 mg/ml (by UV absorbance at 280 nm)

Buffer: "Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide Background(s): Antibodies-Online produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glyci

Lagerung: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Wang, Mao, Lai et al.: "Multiple mesodermal lineage differentiation of Apodemus sylvaticus embryonic stem cells in vitro." in: BMC cell biology, Vol. 11, pp. 42, 2010 (PubMed).