Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) Antikörper

Antigen: Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C)

Synonyme: Cdc25, CDC25C, CDC25, cdc25, xcdc25c

Klonalität: Monoklonal (TC-113)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN97125

Produktbeschreibung

Weitere Bezeichnung: cdc25C

Immunogen: BALB/C mice were injected with purified recombinant CDC25C.

Format: Ig-fraction

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: TC-113

Spezifität: This antibody reacts with a 53-56 kD protein known as CDC25C. The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase. This antibody does not react with mouse and rat.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic Other Applications: Immunoprecipitation, Western Blotting

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Sartor, Ehlert, Grzeschik et al.: "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively." in: Genomics, Vol. 13, Issue 3, pp. 911-2, 1992 (PubMed).

Zwicker, Mueller: "Cell cycle-regulated transcription in mammalian cells." in: Progress in cell cycle research, Vol. 1, pp. 91-9, 1998 (PubMed).