CD105 (Endoglin / TGF-Beta1/3 Receptor) Antikörper

Antigen: CD105 (Endoglin / TGF-Beta1/3 Receptor)

Klonalität: Monoklonal (SN6h)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN97079

Produktbeschreibung

Immunogen: BALB/C mice were injected with purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells.

Format: Ig-fraction

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: SN6h

Spezifität: This antibody reacts with 95 kD (monomer) and 190 kD (dimer) proteins. CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF-beta1 and beta3 with high affinity but not to TGF-beta2.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10 -1:20 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require predigestion with Protease XXXV. Positive Control: Tonsil. Cellular localization: Cell membrane

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: CD Antigene

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with CD105 antibody

Publikationen

Publikationen: Haruta, Seon: "Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 20, pp. 7898-902, 1986 (PubMed).

Nikiteas, Tzanakis, Theodoropoulos et al.: "Vascular endothelial growth factor and endoglin (CD-105) in gastric cancer." in: Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, Vol. 10, Issue 1, pp. 12-7, 2007 (PubMed).