RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1)

Details for Product anti-RAF1 Antibody No. ABIN969559, Anbieter: Anmelden zum Anzeigen
Antigen
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • RAF proto-oncogene serine/threonine-protein kinase pseudogene
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • LOC100341921
  • raf1.S
Reaktivität
Human
831
409
386
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Wirt
Maus
777
62
12
6
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser RAF1 Antikörper ist unkonjugiert
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
508
364
217
172
156
104
52
48
20
18
18
7
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified recombinant fragment of human RAF1 expressed in E. Coli.
Klon 4G4
Isotyp IgG1
Reinigung Purified
Andere Bezeichnung RAF1 (RAF1 Antibody Abstract)
Hintergrund This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Gen-ID 5894
HGNC 5894
Forschungsgebiet Signaling, Kinases/Phosphatases
Pathways MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Applikationshinweise ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS with 0.05 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung 4°C, -20°C for long term storage
Bilder des Herstellers
ELISA image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 2: Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues usin...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 4: Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 5: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recom...
Produkt verwendet in: Hofmann, Heinrich, Radziwill, Radziwil, Moelling: "A short hairpin DNA analogous to miR-125b inhibits C-Raf expression, proliferation, and survival of breast cancer cells." in: Molecular cancer research : MCR, Vol. 7, Issue 10, pp. 1635-44, 2009 (PubMed).

Talmud, Drenos, Shah, Shah, Palmen, Verzilli, Gaunt, Pallas, Lovering, Li, Casas, Sofat, Kumari, Rodriguez, Johnson, Newhouse, Dominiczak, Samani, Caulfield, Sever, Stanton, Shields, Padmanabhan et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. ..." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

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