Maus anti-Human IgG (Heavy & Light Chain) Antikörper

Details zu Produkt Nr. ABIN969481, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
4680
2537
1879
1066
1006
860
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Human
2849
2735
2296
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Wirt
Maus
6705
4831
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Human IgG was isolated from human sera and purified by chromatography.
Klon 4D2D9G8-4A10
Isotyp IgG1
Produktmerkmale Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Reinigung Purified
Hintergrund Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Molekulargewicht 50 kDa
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at 4 °C or at -20 °C for long term.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Human IgG (Heavy & Light Chain) antibody (ABIN969481) Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
Produkt verwendet in: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).