CD63 Antikörper

Antigen: CD63

Klonalität: Monoklonal (NK1-C3)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN96942

Produktbeschreibung

Immunogen: Smooth plasma membrane fraction of MeWo cells.

Format: Ig-fraction

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: NK1-C3

Spezifität: This antibody is specific to 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 to 1:200 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied purified antibody containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: CD Antigene

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human melanoma stained with CD63 antibody

Publikationen

Publikationen: Hagen, Vennegoor, Schlingemann et al.: "Correlation of histopathological characteristics with staining patterns in human melanoma assessed by (monoclonal) antibodies reactive on paraffin sections." in: Histopathology, Vol. 10, Issue 7, pp. 689-700, 1986 (PubMed).

Vennegoor, Ruemke: "Circulating melanoma-associated antigen detected by monoclonal antibody NKI/C-3." in: Cancer immunology, immunotherapy : CII, Vol. 23, Issue 2, pp. 93-100, 1986 (PubMed).

Palmer, Hall, Lew: "A comparison of some methods for identifying amelanotic and oligomelanotic melanoma metastases in paraffin sections." in: Pathology, Vol. 17, Issue 2, pp. 335-9, 1985 (PubMed).