Actin Antikörper

Produktnummer ABIN968903

Produktdetails anti-Actin Antikörper

Target: Actin

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Human, Hund, Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Chicken gizzard muscle Actin

Klon: C4-actin

Isotyp: IgG1 kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Actin Antikörper (ABIN968903)

Yamamori, Itakura, Sugaya, Katsumata, Sakagami, Takahashi: "Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).

Pantaloni, Le Clainche, Carlier: "Mechanism of actin-based motility." in: Science (New York, N.Y.), Vol. 292, Issue 5521, pp. 1502-6, (2001) (PubMed).

Mitchison, Cramer: "Actin-based cell motility and cell locomotion." in: Cell, Vol. 84, Issue 3, pp. 371-9, (1996) (PubMed).

Hanstein, Lange, Schneider-Poetsch, Grolig, Wagner: "Detection of actin and localization of phytochrome in the green alga Mougeotia by monoclonal antibodies." in: Acta histochemica. Supplementband, Vol. 41, Issue 9, pp. 223-30, (1992) (PubMed).

Details zu Actin

Target: Actin

Abstract: Actin Produkte

Synonyme: Act57 antikoerper, Act57A antikoerper, Act57b antikoerper, Actin antikoerper, BEST:RE38067 antikoerper, C antikoerper, CG10067 antikoerper, CG30294 antikoerper, Dmel\\CG10067 antikoerper, M antikoerper, T10 antikoerper, act 57A antikoerper, act57A antikoerper, act57B antikoerper, act57b antikoerper, actin antikoerper, anon-EST:CL2c12 antikoerper, 143060_f_at antikoerper, CG7478 antikoerper, D antikoerper, Dmel\\CG7478 antikoerper, act 79B antikoerper, act79B antikoerper, mus79B antikoerper, Act(88F) antikoerper, Act88-F antikoerper, Act88f antikoerper, CG5178 antikoerper, Dmel\\CG5178 antikoerper, E antikoerper, Ifm(3)7 antikoerper, act 88F antikoerper, act88F antikoerper, rsd antikoerper, Act87e antikoerper, CG18290 antikoerper, Dmel\\CG18290 antikoerper, act 87E antikoerper, act87E antikoerper, mus87E antikoerper, Act88F antikoerper, Dsim\\GD19025 antikoerper, GD19025 antikoerper, dsim_GLEANR_2829 antikoerper, 42A antikoerper, A antikoerper, AFFX-Dros-ACTIN_M_r_at antikoerper, Act antikoerper, Act42 antikoerper, Act42a antikoerper, Actin/BAP47 antikoerper, BAP47 antikoerper, CG12051 antikoerper, Dmel\\CG12051 antikoerper, act 42A antikoerper, act42A antikoerper, cytoplasmic antikoerper, Actin 57B antikoerper, Actin 79B antikoerper, Actin 88F antikoerper, Actin 87E antikoerper, Actin-like protein C08B11.6 antikoerper, hypothetical protein antikoerper, ACT_COLSC Actin (ISS) antikoerper, Actin 42A antikoerper, actin gamma 1 antikoerper, actin antikoerper, Actin, gamma 1 antikoerper, si:ch211-241j12.3 antikoerper, Act57B antikoerper, Act79B antikoerper, Act88F antikoerper, Act87E antikoerper, arp-6 antikoerper, Dsim\Act88F antikoerper, ACTIN antikoerper, Act42A antikoerper, ACTG1 antikoerper, LOC548170 antikoerper, Bm1_16810 antikoerper, IDA5 antikoerper, LOC100342017 antikoerper, si:ch211-241j12.3 antikoerper

Hintergrund: Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments results from interactions between actin and actin-binding proteins. Actin is a 42-kDa protein that is known as G-actin in its monomeric form. Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin. F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks. The latter are highly concentrated beneath the plasma membrane and form the actin cortex. Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins. These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures. Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.

Molekulargewicht: 42 kDa