p38 Antikörper (MAP kinase p38) (pThr180)

Details for Product anti-p38 Antibody No. ABIN968799, Anbieter: Anmelden zum Anzeigen
Antigen
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • mitogen-activated protein kinase 14
  • MAPK14
Epitop
pThr180
67
57
31
15
15
10
10
6
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
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1
Reaktivität
Human, Maus, Ratte (Rattus)
279
219
214
38
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22
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20
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19
19
18
15
15
13
3
1
1
1
Wirt
Maus
196
84
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser p38 Antikörper ist unkonjugiert
7
7
7
7
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3
3
3
3
3
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2
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2
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2
1
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1
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
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69
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48
28
26
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6
2
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Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Human p38 MAPK (pT180/pY182)
Klon 30-p38 MAPK
Isotyp IgG1
Kreuzreaktivität Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung p38 MAPK (p38 Antibody Abstract)
Hintergrund Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-alpha. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAP kinases include p38alpha, beta, gamma, and delta. These ser/thr kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in muliple signal transduction pathways.
Molekulargewicht 38-42 kDa
Forschungsgebiet Kinases/Phosphatases
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling
Kommentare

Related Products: ABIN968535, ABIN968632, ABIN967389, ABIN968769

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.
Bilder des Herstellers
Western Blotting (WB) image for anti-MAP kinase p38 (p38) (pThr180) antibody (ABIN968799) Western blot analysis for p38 MAPK (pT180/pY182) (left figure). HeLa cells (Human cer...
Immunofluorescence (IF) image for anti-MAP kinase p38 (p38) (pThr180) antibody (ABIN968799) Immunofluorescence staining for p38 MAPK (pT180/pY182) (right figure). HeLa cells (Hu...
Produkt verwendet in: Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997 (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996 (PubMed).

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).

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