PCNT Antikörper (AA 1692-1814)

Produktnummer ABIN968666

Produktdetails anti-PCNT Antikörper

Target: PCNT (Pericentrin (PCNT))

Bindungsspezifität: AA 1692-1814

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse pericentrin aa. 1692-1814

Klon: 30-Pericentrin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-PCNT Antikörper (ABIN968666)

Arellano, Guadiana, Breunig, Rakic, Sarkisian: "Development and distribution of neuronal cilia in mouse neocortex." in: The Journal of comparative neurology, Vol. 520, Issue 4, pp. 848-73, (2012) (PubMed).

Young, Dictenberg, Purohit, Tuft, Doxsey: "Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes." in: Molecular biology of the cell, Vol. 11, Issue 6, pp. 2047-56, (2000) (PubMed).

Doxsey, Stein, Evans, Calarco, Kirschner: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, (1994) (PubMed).

Details zu PCNT

Target: PCNT (Pericentrin (PCNT))

Andere Bezeichnung: Pericentrin (PCNT Produkte)

Synonyme: AKAP450 antikoerper, CG13459 antikoerper, CG18648 antikoerper, CG33957 antikoerper, CG6735 antikoerper, CP309 antikoerper, Cp309 antikoerper, D-PLP antikoerper, DPLP antikoerper, Dmel\\CG33957 antikoerper, Dplp antikoerper, PACT antikoerper, PLP antikoerper, Plp antikoerper, d-plp antikoerper, dPLP antikoerper, dplp antikoerper, l(3)s2172 antikoerper, pPlp antikoerper, KEN antikoerper, MOPD2 antikoerper, PCN antikoerper, PCNT2 antikoerper, PCNTB antikoerper, PCTN2 antikoerper, SCKL4 antikoerper, DIP2A antikoerper, AW476095 antikoerper, C86676 antikoerper, Pcnt2 antikoerper, kendrin antikoerper, m239Asp antikoerper, m275Asp antikoerper, pericentrin antikoerper, Pericentrin-like protein antikoerper, pericentrin (kendrin) antikoerper, LOC100055203 antikoerper, Plp antikoerper, PCNT antikoerper, pcnt antikoerper, Pcnt antikoerper

Hintergrund: Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple alpha-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes gamma-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and gamma-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and gamma-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 220 kDa

Pathways: Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interaktom