VTI1B Antikörper (AA 9-121)

Produktnummer ABIN968520

Produktdetails anti-VTI1B Antikörper

Target: VTI1B (Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))

Bindungsspezifität: AA 9-121

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VTI1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Human, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Vti1b aa. 9-121

Klon: 7-Vti1b

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-VTI1B Antikörper (ABIN968520)

Chen, Manga, Orlow: "Pink-eyed dilution protein controls the processing of tyrosinase." in: Molecular biology of the cell, Vol. 13, Issue 6, pp. 1953-64, (2002) (PubMed).

Mallard, Tang, Galli, Tenza, Saint-Pol, Yue, Antony, Hong, Goud, Johannes: "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform." in: The Journal of cell biology, Vol. 156, Issue 4, pp. 653-64, (2002) (PubMed).

Shorter, Beard, Seemann, Dirac-Svejstrup, Warren: "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115." in: The Journal of cell biology, Vol. 157, Issue 1, pp. 45-62, (2002) (PubMed).

Yang, Gonzalez, Prekeris, Steegmaier, Advani, Scheller: "SNARE interactions are not selective. Implications for membrane fusion specificity." in: The Journal of biological chemistry, Vol. 274, Issue 9, pp. 5649-53, (1999) (PubMed).

Advani, Bae, Bock, Chao, Doung, Prekeris, Yoo, Scheller: "Seven novel mammalian SNARE proteins localize to distinct membrane compartments." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10317-24, (1998) (PubMed).

Details zu VTI1B

Target: VTI1B (Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))

Andere Bezeichnung: Vti1b (VTI1B Produkte)

Synonyme: VTI1 antikoerper, VTI1-LIKE antikoerper, VTI1L antikoerper, VTI2 antikoerper, v-SNARE antikoerper, vti1-rp1 antikoerper, AU015348 antikoerper, GES30 antikoerper, MVti1b antikoerper, SNARE antikoerper, Vti1-rp1 antikoerper, zgc:63525 antikoerper, VTI1B antikoerper, vesicle transport through interaction with t-SNAREs 1B antikoerper, vesicle transport through interaction with t-SNAREs 1B pseudogene antikoerper, VTI1B antikoerper, Vti1b antikoerper, vti1b antikoerper, LOC461624 antikoerper

Hintergrund: Eukaryotic protein trafficking involves packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their contents into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs, VAMP1, VAMP2) and target proteins (t-SNAREs, syntaxin 1, SNAP-25) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. With the identification of all SNARE family members in yeast, the research focus has turned to mammalian cells. Here, sequence analysis has identified additional SNARE proteins, including VTI1a and VTI1b. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. VTI1b is a membrane bound protein whose localization overlaps with the cis/medial Golgi marker mannosidase II and the trans-Golgi marker syntaxin 6. VTI1b interacts with, and disrupts the localization of, syntaxin 5. Thus, VTI1b is thought to function in the regulation of post-Golgi vesicle trafficking.

Molekulargewicht: 27 kDa