GIT1 Antikörper (AA 664-770)

Produktnummer ABIN968516

Produktdetails anti-GIT1 Antikörper

Target: GIT1

Bindungsspezifität: AA 664-770

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat GIT1 aa. 664-770

Klon: 13-GIT1

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-GIT1 Antikörper (ABIN968516)

Zhao, Manser, Loo, Lim: "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly." in: Molecular and cellular biology, Vol. 20, Issue 17, pp. 6354-63, (2000) (PubMed).

Premont, Claing, Vitale, Freeman, Pitcher, Patton, Moss, Vaughan, Lefkowitz: "beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 24, pp. 14082-7, (1998) (PubMed).

Details zu GIT1

Target: GIT1

Andere Bezeichnung: GIT1 (GIT1 Produkte)

Synonyme: Cat-1 antikoerper, p95Cat antikoerper, GIT ArfGAP 1 antikoerper, G protein-coupled receptor kinase interacting ArfGAP 1 antikoerper, G protein-coupled receptor kinase-interactor 1 antikoerper, GIT1 antikoerper, Git1 antikoerper

Hintergrund: Activation of adrenergic receptors (AR), such as beta2AR leads to activation of heterotrimeric G proteins and recruitment of G protein-coupled receptor kinases (GRKs). Phosphorylation of the activated receptor by GRKs promotes the binding of arrestin protein which prevents further G protein activation. GRK interactor 1 (GIT1) was identified in a yeast two-hybrid screen for proteins that bind GRK2. GIT1 has an N-terminal Zn2+ finger-like motif involved in GTPase activating activity and multipe ankyrin repeat units. In vitro, GIT1 is a GTPase-activating protein (GAP) for the ADP ribosylation factor (ARF) family of small GTP-binding proteins. In rat, GIT1 is widely expressed with the highest levels in the testis and lowest levels in the liver and spleen. Overexpression of GIT1 in HEK293 cells causes reduced beta2AR signaling and increased receptor phosphorylation as a result of reduced receptor internalization and resensitization. In addition, GIT1 interacts directly with paxillin and the PIX exchange factors, and indirectly with PAKs, Rac1, and Nck. GIT proteins are also now known to be PIP3-stimulated GAPs for ARF6. Thus, GIT1 activation of ARF proteins may involve cell signaling pathways that are important for endocytosis, cell adhesion, and cytoskeletal dynamics.
Synonyms: G protein-coupled receptor kinase interactor 1

Molekulargewicht: 95 kDa