Caveolin Antikörper (pTyr14)

Produktnummer ABIN968480

Produktdetails anti-Caveolin Antikörper

Target: Caveolin (CAV)

Bindungsspezifität: pTyr14

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caveolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Caveolin-1 (Y14) Peptide

Klon: 56-Caveolin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968534

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Caveolin Antikörper (ABIN968480)

Hill, Scherbakov, Schiefermeier, Baran, Hancock, Huber, Parton, Parat: "Reassessing the role of phosphocaveolin-1 in cell adhesion and migration." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 12, pp. 1695-705, (2007) (PubMed).

Labrecque, Royal, Surprenant, Patterson, Gingras, Béliveau: "Regulation of vascular endothelial growth factor receptor-2 activity by caveolin-1 and plasma membrane cholesterol." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 334-47, (2003) (PubMed).

Lee, Volonte, Galbiati, Iyengar, Lublin, Bregman, Wilson, Campos-Gonzalez, Bouzahzah, Pestell, Scherer, Lisanti: "Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: identification of a c-Src/Cav-1/Grb7 signaling cassette." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 11, pp. 1750-75, (2001) (PubMed).

Ushio-Fukai, Hilenski, Santanam, Becker, Ma, Griendling, Alexander: "Cholesterol depletion inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells: role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48269-75, (2001) (PubMed).

Volonté, Galbiati, Pestell, Lisanti: "Cellular stress induces the tyrosine phosphorylation of caveolin-1 (Tyr(14)) via activation of p38 mitogen-activated protein kinase and c-Src kinase. Evidence for caveolae, the actin cytoskeleton, and focal adhesions as mechanical sensors of osmotic stres" in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8094-103, (2001) (PubMed).

Glenney, Soppet: "Sequence and expression of caveolin, a protein component of caveolae plasma membrane domains phosphorylated on tyrosine in Rous sarcoma virus-transformed fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10517-21, (1992) (PubMed).

Details zu Caveolin

Target: Caveolin (CAV)

Andere Bezeichnung: Caveolin

Hintergrund: Caveolin (VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. In addition, it is present in the trans-Golgi network (TGN) and in apically and basolaterally destined transport vesicles. Caveolin is a transmembrane adaptor molecule that recognizes GPI-linked proteins and interacts with downstream cytoplasmic signaling molecules, such as src-family tyrosine kinases and hetero-trimeric G proteins. Caveolin forms large lipid-binding oligomers, which are thought to play a role in caveolae formation. It may also function as a scaffolding protein, which organizes signaling molecules. This functional role is supported by the fact that caveolin interacts directly with inactive ras and G-protein alpha subunits. Phosphorylation of caveolin at Tyr-14, Ser-88, and other residues in v-src transformed cells leads to flattening, aggregation, and fusion of caveolae and caveolae-derived vesicles. Thus, caveolin is the principle protein of caveolae and may be involved in v-src mediated cellular transformation.
This antibody has also been reported to cross-react to paxillin in mouse embryonic fibroblasts (MEF), observable to migrate at ~ 68 kDa .

Molekulargewicht: 21-24 kDa