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PSME3 Antikörper (AA 45-147)

PSME3 Reaktivität: Human, Ratte, Maus, Hund WB, BI Wirt: Maus Monoclonal 47-Psme3 unconjugated
Produktnummer ABIN968402
  • Target Alle PSME3 Antikörper anzeigen
    PSME3
    Bindungsspezifität
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 45-147
    Reaktivität
    • 41
    • 20
    • 15
    • 8
    • 7
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Ratte, Maus, Hund
    Wirt
    • 38
    • 6
    Maus
    Klonalität
    • 40
    • 4
    Monoklonal
    Konjugat
    • 29
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PSME3 Antikörper ist unkonjugiert
    Applikation
    • 30
    • 14
    • 12
    • 8
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), BioImaging (BI)
    Kreuzreaktivität
    Ratte (Rattus), Hund, Human
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    6. Triton is a trademark of the Dow Chemical Company.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse Psme3/PA28-gamma aa. 45-147
    Klon
    47-Psme3
    Isotyp
    IgG1
    Top Product
    Discover our top product PSME3 Primärantikörper
  • Applikationshinweise
    Bioimaging
    1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
    2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
    3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
    4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
    5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
    6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
    7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
    8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
    9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
    10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    11. View and analyze the cells on an appropriate imaging instrument.
    Kommentare

    Related Products: ABIN967389, ABIN968545

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store undiluted at -20°C.
  • Kohda, Ishibashi, Shimbara, Tanaka, Matsuda, Kasahara et al.: "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4923-35, (1998) (PubMed).

  • Target
    PSME3
    Andere Bezeichnung
    Psme3 (PSME3 Produkte)
    Synonyme
    AA410043 antikoerper, AU020960 antikoerper, Ki antikoerper, PA28gamma antikoerper, REGgamma antikoerper, pa28g antikoerper, Ab2-371 antikoerper, PA28-gamma antikoerper, PA28G antikoerper, REG-GAMMA antikoerper, psme3b antikoerper, proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) antikoerper, proteasome activator subunit 3 antikoerper, proteasome activator subunit 3 S homeolog antikoerper, Psme3 antikoerper, PSME3 antikoerper, psme3.S antikoerper
    Hintergrund
    The 20S proteasome is the major constituent of the proteasomal complex that mediates degradation events, including the generation of antigenic peptides that interact with MHC class I. The 20S proteasome is a cylindrical shaped complex composed of four layers of rings, each with seven subunits. The inner rings contain alpha-subunits, while the outer rings contain beta-catalytic subunits. The constituents of the PA28 activator complex are additional subunits with specialized roles in class I-mediated antigen presentation. PA28 is a ring-shaped structure with alternating alpha- and beta-subunits. This complex binds the alpha-rings of 20S and stimulates its activity. Expression of the PA28 alpha- and beta-subunits is strongly induced by IFN-gamma. The protein product of the Psme3 gene, also known as the Ki antigen, is highly homologous to the PA28 alpha- and beta-subunits and has been designated the PA28 gamma-subunit. This protein forms a homohexamer that binds the 20S proteasome and is thought to modulate proteasome activity. The PA28 alpha- and beta-subunits are located in the cytoplasm and in the nucleus, while the gamma-subunit is almost exclusively nuclear.
    Synonyms: PA28-gamma
    Molekulargewicht
    36 kDa
    Pathways
    Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA
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