MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290)

Details for Product anti-FRAP1 Antibody No. ABIN968383, Anbieter: Anmelden zum Anzeigen
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternativen
anti-Human MTOR Antikörper für Immunofluorescence (fixed cells)
Epitop
AA 185-290
64
28
26
22
21
20
15
15
12
10
9
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Human, Maus, Ratte (Rattus)
368
200
198
18
9
8
7
7
7
6
4
2
2
2
1
1
Wirt
Maus
317
47
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Immunofluorescence (IF), Western Blotting (WB)
246
111
94
73
54
52
37
20
7
7
7
6
3
2
2
2
1
1
1
Optionen
Hersteller
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Immunogen Rat RAFT1 aa. 185-290
Klon 30-RAFT1
Isotyp IgG1
Kreuzreaktivität Maus, Human, Huhn
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung RAFT1 (FRAP1 Antibody Abstract)
Hintergrund Immunosuppressive agents such as cyclosporin A, FK506, and rapamycin are used routinely in clinical transplantation and the treatment of autoimmune disorders. Cyclosporin A and FK506 bind to cyclophilins and the FK-binding protein FKBP12, respectively. These complexes interact with, and inhibit the activity of, the calcineurin protein phosphatase, which is essential for T cell activation. Rapamycin also interacts with FKBP12, but this complex does not affect calcineurin. Instead, it inhibits signals required for G1 to S progression in yeast, in IL-2-stimulated T lymphocytes, and other mammalian cell types. Rapamycin-FKBP12 binds to a protein designated Rapamycin And FKBP12 Target-1 (RAFT1) in rats and FKBP-Rapamycin-Associated Protein (FRAP) in humans. RAFT1 is the homolog of the yeast genes TOR1 and TOR2 which, when mutated, lead to rapamycin resistance. RAFT1 is a PI4-kinase that exhibits homology with other PI kinases such as PI3-kinase and PI4-Kalpha. RAFT1 phosphorylates p70 S6 kinase and 4E-BP1 which are regulators of translational initiation. However, rapamycin-FKBP1 does not inhibit PI4-kinase activity and, therefore, affects RAFT1 activity via alternate mechanisms.
Synonyms: Rapamycin And FKBP12 Target-1
Molekulargewicht 245 kDa
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Kommentare

Related Products: ABIN968539, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.
Bilder des Herstellers
Western Blotting (WB) image for anti-MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290) (ABIN968383) Western blot analysis of RAFT1 on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL-172...
Immunofluorescence (IF) image for anti-MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290) (ABIN968383) Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).
Produkt verwendet in: Castedo, Ferri, Blanco, Roumier, Larochette, Barretina, Amendola, Nardacci, Métivier, Este, Piacentini, Kroemer: "Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation." in: The Journal of experimental medicine, Vol. 194, Issue 8, pp. 1097-110, 2001 (PubMed).

Alarcon, Cardenas, Heitman: "Mammalian RAFT1 kinase domain provides rapamycin-sensitive TOR function in yeast." in: Genes & development, Vol. 10, Issue 3, pp. 279-88, 1996 (PubMed).

Sabatini, Pierchala, Barrow, Schell, Snyder: "The rapamycin and FKBP12 target (RAFT) displays phosphatidylinositol 4-kinase activity." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20875-8, 1995 (PubMed).

Allgemeine Veröffentlichungen Burnett, Barrow, Cohen, Snyder, Sabatini: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998 (PubMed).

Sabatini, Erdjument-Bromage, Lui, Tempst, Snyder: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994 (PubMed).