PDPK1 Antikörper (3-phosphoinositide Dependent Protein Kinase-1) (AA 439-555)

Details for Product anti-PDPK1 Antibody No. ABIN968352, Anbieter: Anmelden zum Anzeigen
Antigen
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • si:zfos-979f1.3
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3'-phosphoinositide-dependent protein kinase 1
  • 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1
  • ATPDK1
  • AtPDK1
  • T32M21.110
  • T32M21_110
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1 L homeolog
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide-dependent protein kinase 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • pyruvate dehydrogenase kinase 1
  • Phosphoinositide-dependent kinase 1
  • 3'-phosphoinositide-dependent protein kinase 1
  • PDPK1
  • pdpk1.L
  • pdpk1
  • Pdpk1
  • LOC100380750
  • pdk1
  • PDK1
  • Pdk1
  • pdk-1
Epitop
AA 439-555
58
35
32
27
27
26
18
17
15
15
15
15
7
7
6
6
6
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
462
218
218
19
13
12
11
10
6
5
4
3
2
1
1
1
1
1
1
1
Wirt
Maus
382
72
16
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser PDPK1 Antikörper ist unkonjugiert
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
311
158
100
91
67
63
40
31
22
10
7
5
3
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Rat PDPK1 aa. 439-555
Klon 5-PDK1
Isotyp IgG1
Kreuzreaktivität Human, Hund, Maus
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung PDK1 (PDPK1 Antibody Abstract)
Hintergrund Protein Kinase B (PKB) is downstream of phosphoinositide 3-kinase (PI3-Kinase) that functions in a critical signaling pathway for receptor-dependent cell activation. PKB activation requires the binding of lipid metabolites phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate, phospholipid products of PI3-Kinase. These lipids bind to PKB and induce a conformational change in the enzyme, resulting in its translocation to the plasma membrane. Following cell activation, PKB is phosphorylated on Thr-308 by another kinase designated as 3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) or also known as PKB Kinase. This kinase probably represents a new family of phospholipid-dependent activities with molecular weights that range from 31 to 220 kDa. PDPK1 consists of 559 amino acids with an N-terminal catalytic domain and a pleckstrin-homology (PH) domain located in the C-terminal region. Like PKB, PDPK1 requires the presence of phosphatidylinositol 3,4,5-trisphosphate for maximal activity. Thus, PDPK1 acts upstream of PKB and may control signals important for proliferation, apoptosis, and metabolism. This antibody is routinely tested by western blot analysis.
Synonyms: PKB Kinase, Akt Kinase, PDK1
Molekulargewicht 58-63 kDa
Forschungsgebiet Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Kommentare

Related Products: ABIN968552, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.
Bilder des Herstellers
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 439-555) antibody (ABIN968352) Western blot analysis of PDPK1 on a SW13 cell lysate (human adrenal gland carcinoma, ...
 image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 439-555) antibody (ABIN968352) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 439-555) antibody (Image 2)
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 439-555) antibody (ABIN968352) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 439-555) antibody (Image 3)
Produkt verwendet in: Fujita, Sato, Ishida, Tsuruo: "Involvement of Hsp90 in signaling and stability of 3-phosphoinositide-dependent kinase-1." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10346-53, 2002 (PubMed).

Scheid, Marignani, Woodgett: "Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6247-60, 2002 (PubMed).

Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000 (PubMed).

Klippel, Kavanaugh, Pot, Williams: "A specific product of phosphatidylinositol 3-kinase directly activates the protein kinase Akt through its pleckstrin homology domain." in: Molecular and cellular biology, Vol. 17, Issue 1, pp. 338-44, 1997 (PubMed).

Stokoe, Stephens, Copeland, Gaffney, Reese, Painter, Holmes, McCormick, Hawkins: "Dual role of phosphatidylinositol-3,4,5-trisphosphate in the activation of protein kinase B." in: Science (New York, N.Y.), Vol. 277, Issue 5325, pp. 567-70, 1997 (PubMed).

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