LAMP1 Antikörper (AA 25-224)

Produktnummer ABIN968337

Produktdetails anti-LAMP1 Antikörper

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Bindungsspezifität: AA 25-224

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Lamp-1 aa. 25-224

Klon: 25-Lamp

Isotyp: IgG2b

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-LAMP1 Antikörper (ABIN968337)

Alto, Soderling, Scott: "Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics." in: The Journal of cell biology, Vol. 158, Issue 4, pp. 659-68, (2002) (PubMed).

Hocking, Kowalski: "A cryptic fragment from fibronectin's III1 module localizes to lipid rafts and stimulates cell growth and contractility." in: The Journal of cell biology, Vol. 158, Issue 1, pp. 175-84, (2002) (PubMed).

Spoerl, Stumpf, Noegel, Hasse: "Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48858-67, (2002) (PubMed).

Fukuda: "Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking." in: The Journal of biological chemistry, Vol. 266, Issue 32, pp. 21327-30, (1991) (PubMed).

Fukuda, Viitala, Matteson, Carlsson: "Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences." in: The Journal of biological chemistry, Vol. 263, Issue 35, pp. 18920-8, (1989) (PubMed).

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: Lamp-1 (LAMP1 Produkte)

Synonyme: CD107a antikoerper, LAMPA antikoerper, LGP120 antikoerper, AI196048 antikoerper, Lamp-1 antikoerper, LEP100 antikoerper, LAMP-1 antikoerper, LGP-A antikoerper, Lgpa antikoerper, Lysosome-associated membrane protein 1 antikoerper, cd107a antikoerper, lamp-1 antikoerper, lamp1 antikoerper, lampa antikoerper, lgp120 antikoerper, rexp76 antikoerper, lysosomal associated membrane protein 1 antikoerper, lysosomal-associated membrane protein 1 antikoerper, lysosomal-associated membrane protein 1 L homeolog antikoerper, LAMP1 antikoerper, Lamp1 antikoerper, lamp1.L antikoerper

Hintergrund: Lysosomes are digestive compartments in mammalian cells that are responsible for the degradation of numerous substances including foreign materials. The lysosomal membrane is of interest due to its role in maintaining the acidic intralysosomal sac, its resistance to degradation, and its ability to fuse with other membranous organelles. Lamp-1 and lamp-2 (lysosomal-associated membrane proteins -1 and -2) were identified as lysosomal membrane proteins that act as carriers for poly-N-glycans, primarily N-acetyllactosamines. They consist of two heavily glycosylated amino terminal domains, a single trans-membrane domain, and a short cytoplasmic tail. A tyrosine located at the fourth residue from the end of the cytoplasmic tail, a large hydrophobic amino acid as the last residue, and a glycine residue amino terminal to the tyrosine residue targets these proteins from the TGN to the lysosome. The role of the Lamp molecules may be a carrier for poly-N-acetyllactosamines and/or to protect lysosomal membranes from the soluble hydrolases. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 110 kDa

Pathways: Autophagie