TJP1 Antikörper (Tight Junction Protein 1 (Zona Occludens 1)) (AA 1048-1247)

Details for Product anti-TJP1 Antibody No. ABIN968282, Anbieter: Anmelden zum Anzeigen
Antigen
  • ZO-1
  • ZO1
  • zo1
  • tight junction protein 1
  • tight junction protein 1 (zona occludens 1)
  • zonula occludens 1
  • TJP1
  • Tjp1
  • ZO1
Epitop
AA 1048-1247
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10
5
4
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3
3
2
2
2
2
2
2
2
1
1
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Reaktivität
Human
94
35
33
20
11
5
4
4
3
2
1
1
1
1
Wirt
Maus
77
16
3
2
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser TJP1 Antikörper ist unkonjugiert
3
2
2
2
1
1
1
1
1
1
1
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1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
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26
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14
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11
10
9
8
3
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Human ZO-1 aa. 1048-1247
Klon 1-ZO
Isotyp IgG1
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung ZO-1 (TJP1 Antibody Abstract)
Hintergrund Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intracellular barriers between epithelial and endothelial cells. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1, and ZO-2. Analysis of ZO-1 and -2 resulted in their inclusion in the MAGUK protein family. This family also includes the discs large tumor suppressor protein (Dlg-A) of Drosophila, p55, an erythrocyte membrane protein, and PSD-95/SAP90, a synaptic membrane protein. All family members contain a region homologous to guanylate kinase (GuK), a src homology (SH3) domain, and multiple PDZ domains. Through these elements, MAGUK proteins function in signal transduction and, possibly, tumor suppression. ZO-1 is a peripheral membrane phosphoprotein that binds to other tight junction proteins such as occludin and AF-6. Via its SH3 domain, ZO-1 interacts with a serine protein kinase that phosphorylates a region immediately C-terminal of the SH3 domain. Taken together, these data indicate that ZO-1 is a critical element in the formation of tight junctions and may also serve an important role in signaling and tumor suppression. This antibody is routinely tested by western blot analysis.
Molekulargewicht 220 kDa
Pathways Carbohydrate Homeostasis, Cell-Cell Junction Organization
Kommentare

Related Products: ABIN968535, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.
Bilder des Herstellers
Western Blotting (WB) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody (ABIN968282) Western blot analysis of ZO-1 on a HeLa cell lysate. Lane 1: 1:250, lane 2: 1:500, la...
Immunofluorescence (IF) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody (ABIN968282) Immunofluorescent staining of EAHY human endothelial cells.
Produkt verwendet in: Hirase, Kawashima, Wong, Ueyama, Rikitake, Tsukita, Yokoyama, Staddon: "Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 10423-31, 2001 (PubMed).

Nix, Chishti, Anderson, Walther: "hCASK and hDlg associate in epithelia, and their src homology 3 and guanylate kinase domains participate in both intramolecular and intermolecular interactions." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41192-200, 2001 (PubMed).

Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, 2000 (PubMed).

Willott, Balda, Fanning, Jameson, Van Itallie, Anderson: "The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 16, pp. 7834-8, 1993 (PubMed).

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