MSH6 Antikörper (MutS Homolog 6 (E. Coli)) (AA 225-333)

Details for Product anti-MSH6 Antibody No. ABIN968247, Anbieter: Anmelden zum Anzeigen
Antigen
  • cb326
  • wu:fb36c12
  • LOC100227810
  • GTBP
  • GTMBP
  • HNPCC5
  • HSAP
  • p160
  • AU044881
  • AW550279
  • Gtmbp
  • mutS homolog 6 (E. coli)
  • LOC100360342
  • msh6
  • Msh6
  • MSH6
  • LOC100227810
Epitop
AA 225-333
18
5
5
4
4
4
4
3
2
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
151
50
39
8
7
2
1
1
1
1
1
Wirt
Maus
78
61
12
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser MSH6 Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
106
51
46
42
37
13
13
12
9
6
5
4
4
3
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Human MSH6 aa. 225-333
Klon 44-MSH6
Isotyp IgG1
Kreuzreaktivität Hund, Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung MSH6 (MSH6 Antibody Abstract)
Hintergrund DNA mismatch repair in bacteria is carried out by the MutL, MutH, and MutS proteins. Initial binding of MutS to the mismatched DNA is followed by binding of the MutH endonuclease and MutL. Together these proteins form a complex that mediates excision repair. Mutations or deficiencies of any of these bacterial proteins results in a mutator phenotype that is characterized by genetic instability. MSH2, MSH3, and MSH6 are human homologs of MutS, while MLH1, PMS1, and PMS2 are homologs of MutL. As a heterodimer with MSH2, MSH6 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes single-base mispairs and insertion/deletion loops. Binding of this complex induces conformational changes in the DNA that lead to the binding of an MLH-PMS1 complex and excision repair. Mutations in the human genes are associated with hereditary nonpolyposis colon cancer (HNPCC), a common hereditary disease in humans. HNPCC is characterized by frequent microsatellite mutations that arise from somatic mutation due to a replication error (RER+) phenotype. This phenotype is analogous to the bacterial system and is directly linked to DNA mismatch repair deficiencies.
Synonyms: GTBP
Molekulargewicht 160 kDa
Forschungsgebiet Chromatin and Nuclear Signaling, DNA/RNA
Pathways DNA Reparatur, Chromatin Binding, Production of Molecular Mediator of Immune Response
Kommentare

Related Products: ABIN968533, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.
Bilder des Herstellers
Western Blotting (WB) image for anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 225-333) antibody (ABIN968247) Western blot analysis of MSH6/GTBP on A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lan...
Immunofluorescence (IF) image for anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 225-333) antibody (ABIN968247) Immunofluorescent staining of C3H10T1/2 cells.
Produkt verwendet in: Kariola, Otway, Lönnqvist, Raevaara, Macrae, Vos, Kohonen-Corish, Hofstra, Nyström-Lahti: "Two mismatch repair gene mutations found in a colon cancer patient--which one is pathogenic?" in: Human genetics, Vol. 112, Issue 2, pp. 105-9, 2003 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Humbert, Hermine, Hernandez, Bouget, Selves, Laurent, Salles, Lautier: "Implication of protein kinase C in the regulation of DNA mismatch repair protein expression and function." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18061-8, 2002 (PubMed).

Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, 2000 (PubMed).

Palombo, Gallinari, Iaccarino, Lettieri, Hughes, DArrigo, Truong, Hsuan, Jiricny: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." in: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, 1995 (PubMed).

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