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PRKAR2B Antikörper (AA 1-418)

PRKAR2B Reaktivität: Human, Maus, Ratte, Huhn, Hund WB, IHC, IF, IP Wirt: Maus Monoclonal 45 unconjugated
Produktnummer ABIN968072
  • Target Alle PRKAR2B Antikörper anzeigen
    PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
    Bindungsspezifität
    • 11
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 1-418
    Reaktivität
    • 61
    • 32
    • 22
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Huhn, Hund
    Wirt
    • 55
    • 6
    Maus
    Klonalität
    • 54
    • 7
    Monoklonal
    Konjugat
    • 32
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser PRKAR2B Antikörper ist unkonjugiert
    Applikation
    • 50
    • 38
    • 11
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Kreuzreaktivität
    Huhn, Hund, Maus, Ratte (Rattus)
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human PKA RIIbeta, aa 1-418
    Klon
    45
    Isotyp
    IgG1
    Top Product
    Discover our top product PRKAR2B Primärantikörper
  • Applikationshinweise
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    Kommentare

    Related Products: ABIN967389

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store undiluted at -20° C.
  • Kranz, Dorgau, Pottek, Herrling, Schultz, Bolte, Monyer, Penuela, Laird, Dedek, Weiler, Janssen-Bienhold: "Expression of Pannexin1 in the outer plexiform layer of the mouse retina and physiological impact of its knockout." in: The Journal of comparative neurology, Vol. 521, Issue 5, pp. 1119-35, (2013) (PubMed).

    Puller, Ondreka, Haverkamp: "Bipolar cells of the ground squirrel retina." in: The Journal of comparative neurology, Vol. 519, Issue 4, pp. 759-74, (2011) (PubMed).

    Hilgen, von Maltzahn, Willecke, Weiler, Dedek: "Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina." in: The Journal of comparative neurology, Vol. 519, Issue 3, pp. 433-50, (2011) (PubMed).

    Haverkamp, Specht, Majumdar, Zaidi, Brandstätter, Wasco, Wässle, Tom Dieck: "Type 4 OFF cone bipolar cells of the mouse retina express calsenilin and contact cones as well as rods." in: The Journal of comparative neurology, Vol. 507, Issue 1, pp. 1087-101, (2008) (PubMed).

    Mataruga, Kremmer, Müller: "Type 3a and type 3b OFF cone bipolar cells provide for the alternative rod pathway in the mouse retina." in: The Journal of comparative neurology, Vol. 502, Issue 6, pp. 1123-37, (2007) (PubMed).

    Tavalin, Colledge, Hell, Langeberg, Huganir, Scott: "Regulation of GluR1 by the A-kinase anchoring protein 79 (AKAP79) signaling complex shares properties with long-term depression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 8, pp. 3044-51, (2002) (PubMed).

    Taskén, Collas, Kemmner, Witczak, Conti, Taskén: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, (2001) (PubMed).

    Casey, Vaughan, He, Hatcher, Winter, Weremowicz, Montgomery, Kucherlapati, Morton, Basson: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, (2000) (PubMed).

    Skålhegg, Landmark, Foss, Lohmann, Hansson, Lea, Jahnsen: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, (1992) (PubMed).

    Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).

  • Target
    PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
    Andere Bezeichnung
    PKA RIIbeta (PRKAR2B Produkte)
    Synonyme
    MGC83177 antikoerper, PRKAR2B antikoerper, AI451071 antikoerper, AW061005 antikoerper, PKARIIbeta antikoerper, Pkarb2 antikoerper, RII(beta) antikoerper, RATDNA antikoerper, PRKAR2 antikoerper, RII-BETA antikoerper, protein kinase, cAMP-dependent, regulatory subunit type II beta S homeolog antikoerper, protein kinase cAMP-dependent type II regulatory subunit beta antikoerper, protein kinase, cAMP-dependent, regulatory, type II, beta antikoerper, protein kinase, cAMP dependent regulatory, type II beta antikoerper, protein kinase cAMP-dependent type 2 regulatory subunit beta antikoerper, prkar2b.S antikoerper, PRKAR2B antikoerper, prkar2b antikoerper, Prkar2b antikoerper
    Hintergrund
    CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIß, RIIalpha, and RIIß.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cß, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rß isoforms are predominant in nervous and adipose tissues. There are indications that the deletion of the gene for PKA RIIß results in lack of long-term potentiation in a select group of hippocampal cells, suggesting an important role for this protein in the neurosciences.
    Molekulargewicht
    53 kDa
    Pathways
    Hedgehog Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion
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