IQGAP1 Antikörper (AA 1348-1490)

Produktnummer ABIN968063

Produktdetails anti-IQGAP1 Antikörper

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Bindungsspezifität: AA 1348-1490

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IQGAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Ratte (Rattus), Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IQGAP1 aa. 1348-1490

Klon: 24-IQGAP1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-IQGAP1 Antikörper (ABIN968063)

Mbele, Deloulme, Gentil, Delphin, Ferro, Garin, Takahashi, Baudier: "The zinc- and calcium-binding S100B interacts and co-localizes with IQGAP1 during dynamic rearrangement of cell membranes." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49998-50007, (2002) (PubMed).

Ruiz-Velasco, Lanning, Williams: "The activation of Rac1 by M3 muscarinic acetylcholine receptors involves the translocation of Rac1 and IQGAP1 to cell junctions and changes in the composition of protein complexes containing Rac1, IQGAP1, and actin." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33081-91, (2002) (PubMed).

Li, Wang, Chakladar, Bronson, Bernards: "Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1." in: Molecular and cellular biology, Vol. 20, Issue 2, pp. 697-701, (2000) (PubMed).

Hart, Callow, Souza, Polakis: "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs." in: The EMBO journal, Vol. 15, Issue 12, pp. 2997-3005, (1996) (PubMed).

Kuroda, Fukata, Kobayashi, Nakafuku, Nomura, Iwamatsu, Kaibuchi: "Identification of IQGAP as a putative target for the small GTPases, Cdc42 and Rac1." in: The Journal of biological chemistry, Vol. 271, Issue 38, pp. 23363-7, (1996) (PubMed).

Details zu IQGAP1

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Andere Bezeichnung: IQGAP1 (IQGAP1 Produkte)

Synonyme: HUMORFA01 antikoerper, SAR1 antikoerper, p195 antikoerper, AA682088 antikoerper, D7Ertd237e antikoerper, D7Ertd257e antikoerper, mKIAA0051 antikoerper, IQ motif containing GTPase activating protein 1 antikoerper, IQGAP1 antikoerper, Iqgap1 antikoerper

Hintergrund: GTPases (Ras, Rho, cdc42Hs, and rac) modulate various cellular functions such as cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis (GTPases). IQGAP1 is Ras-GAP related protein that contains a WW domain that binds poly-proline regions, a calponin-homology domain (CHD), an IQ domain of unconventional myosins, and a C-terminal GAP-related domain (GRD). IQGAP1 co-immunoprecipitates with cdc42 and calmodulin, and colocalizes with cdc42Hs at the leading edges of the plasma membrane and at the cell-cell junctions. IQGAP1 may interact with both cadherins and catenins leading to dissociation of these proteins from sites of cell adhesion. Thus, IQGAP1 may be a Rac1 and Cdc42 effector that negatively regulates cadherin-mediated cell junctions.

Molekulargewicht: 195 kDa

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2