CaM Kinase Kinase (AA 341-504) Antikörper

Produktnummer ABIN968029

Produktdetails

Target: CaM Kinase Kinase

Bindungsspezifität: AA 341-504

Reaktivität: Hund, Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Human, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat CaM Kinase Kinase aa. 341-504

Klon: 6-CaM Kinase Kinase

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen

Egea, Espinet, Soler, Dolcet, Yuste, Encinas, Iglesias, Rocamora, Comella: "Neuronal survival induced by neurotrophins requires calmodulin." in: The Journal of cell biology, Vol. 154, Issue 3, pp. 585-97, (2001) (PubMed).

Wayman, Walters, Kolibaba, Soderling, Christian: "CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 811-24, (2000) (PubMed).

Wu, Means: "Ca(2+)/calmodulin-dependent protein kinase IV is expressed in spermatids and targeted to chromatin and the nuclear matrix." in: The Journal of biological chemistry, Vol. 275, Issue 11, pp. 7994-9, (2000) (PubMed).

Edelman, Mitchelhill, Selbert, Anderson, Hook, Stapleton, Goldstein, Means, Kemp: "Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca(2+)-calmodulin, and partial amino acid sequence." in: The Journal of biological chemistry, Vol. 271, Issue 18, pp. 10806-10, (1996) (PubMed).

Tokumitsu, Enslen, Soderling: "Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase." in: The Journal of biological chemistry, Vol. 270, Issue 33, pp. 19320-4, (1995) (PubMed).

Antigendetails

Target: CaM Kinase Kinase

Hintergrund: Ca2+/Calmodulin (CaM)-dependent kinases are multifunctional kinases involved in a myriad of cellular functions such as neurotransmitter synthesis, long-term potentiation, and formation of spatial learning. A 68kDa CaM Kinase Kinase with homology to the Ca2+/CaM-dependent kinases, phosphorylates and activates Ca2+/CaM-dependent protein kinases I and IV, but not type II. This phosphorylation occurs on Ser/Thr residues and is Ca2+ and CaM dependent. CaM Kinase Kinase is abundant in brain tissue, and is thought to be an upstream regulator in the Ca2+/CaM-dependent neural processes. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 68 kDa