EEA1 Antikörper (AA 3-281)

Produktnummer ABIN967983

Produktdetails anti-EEA1 Antikörper

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Bindungsspezifität: AA 3-281

Reaktivität: Human, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EEA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Ratte (Rattus), Huhn, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EEA1 aa. 3-281

Klon: 14-EEA1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-EEA1 Antikörper (ABIN967983)

van Dam, Stoorvogel: "Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 169-82, (2002) (PubMed).

Yan, Han, Miao, Greengard, Xu: "The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36788-96, (2001) (PubMed).

Allan, Moyer, Balch: "Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion." in: Science (New York, N.Y.), Vol. 289, Issue 5478, pp. 444-8, (2000) (PubMed).

Mullock, Smith, Ihrke, Bright, Lindsay, Parkinson, Brooks, Parton, James, Luzio, Piper: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3137-53, (2000) (PubMed).

Mu, Callaghan, Steele-Mortimer, Stenmark, Parton, Campbell, McCluskey, Yeo, Tock, Toh: "EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13503-11, (1995) (PubMed).

Details zu EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Andere Bezeichnung: EEA1 (EEA1 Produkte)

Synonyme: MST105 antikoerper, MSTP105 antikoerper, ZFYVE2 antikoerper, im:7151048 antikoerper, si:ch211-276p10.2 antikoerper, A430109M19Rik antikoerper, B230358H09Rik antikoerper, early endosome antigen 1 antikoerper, early endosome antigen 1 S homeolog antikoerper, EEA1 antikoerper, eea1 antikoerper, Eea1 antikoerper, eea1.S antikoerper

Hintergrund: Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly alpha-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine finger motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 180 kDa

Pathways: Toll-Like Receptors Cascades