Gelsolin Antikörper (AA 592-768)

Produktnummer ABIN967953

Produktdetails anti-Gelsolin Antikörper

Target: Gelsolin (GSN)

Bindungsspezifität: AA 592-768

Reaktivität: Human, Maus, Ratte, Kaninchen, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Gelsolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Maus, Kaninchen, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Gelsolin aa. 592-768

Klon: 2-Gelsolin

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Gelsolin Antikörper (ABIN967953)

Wang, Xie, Du, Wu, Feng, Mei, McDonald, Xiong: "Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin." in: The Journal of cell biology, Vol. 160, Issue 4, pp. 565-75, (2003) (PubMed).

Arnt, Chiorean, Heldebrant, Gores, Kaufmann: "Synthetic Smac/DIABLO peptides enhance the effects of chemotherapeutic agents by binding XIAP and cIAP1 in situ." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44236-43, (2002) (PubMed).

De Botton, Sabri, Daugas, Zermati, Guidotti, Hermine, Kroemer, Vainchenker, Debili: "Platelet formation is the consequence of caspase activation within megakaryocytes." in: Blood, Vol. 100, Issue 4, pp. 1310-7, (2002) (PubMed).

Slee, Adrain, Martin: "Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7320-6, (2001) (PubMed).

Kwiatkowski, Stossel, Orkin, Mole, Colten, Yin: "Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain." in: Nature, Vol. 323, Issue 6087, pp. 455-8, (1986) (PubMed).

Details zu Gelsolin

Target: Gelsolin (GSN)

Andere Bezeichnung: Gelsolin (GSN Produkte)

Synonyme: ADF antikoerper, AGEL antikoerper, CG1106 antikoerper, DGS antikoerper, Dmel\\CG1106 antikoerper, gel antikoerper, scin antikoerper, cb107 antikoerper, gsn antikoerper, sb:cb107 antikoerper, u-gelsolin antikoerper, wu:fi16f06 antikoerper, gelsolin antikoerper, Gelsolin antikoerper, gelsolin S homeolog antikoerper, gelsolin a antikoerper, GSN antikoerper, Gel antikoerper, Gsn antikoerper, gsn.S antikoerper, gsn antikoerper, gsna antikoerper

Hintergrund: Gelsolin was identified as a result of its ability to sever actin filaments in a Ca2+-dependent manner. The gene for gelsolin encodes an 83 kDa protein that migrates as a 93 kDa polypeptide in SDS-gels. The N-terminal domain contains the calcium-independent actin-severin site, whereas the calcium-dependent site is located in the C-terminal portion of the protein. It exhibits significant homology with villin, another calcium-regulated actin filament severing protein. Gelsolin can be found intracellularly, as well as in a secreted form. However, both forms are encoded by the same gene. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 93 kDa

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, Autophagie