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AP2B1 Antikörper (AA 75-245)

AP2B1 Reaktivität: Human, Maus, Ratte, Hund, Huhn, Frosch WB, IHC, IP, BI Wirt: Maus Monoclonal 74-Adaptin beta unconjugated
Produktnummer ABIN967930
  • Target Alle AP2B1 Antikörper anzeigen
    AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))
    Bindungsspezifität
    • 15
    • 8
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 75-245
    Reaktivität
    • 62
    • 22
    • 17
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    Human, Maus, Ratte, Hund, Huhn, Frosch
    Wirt
    • 59
    • 3
    Maus
    Klonalität
    • 59
    • 3
    Monoklonal
    Konjugat
    • 31
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser AP2B1 Antikörper ist unkonjugiert
    Applikation
    • 38
    • 24
    • 13
    • 13
    • 8
    • 6
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)
    Kreuzreaktivität
    Maus, Ratte (Rattus), Hund, Huhn, Frosch
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Please refer to us for technical protocols.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Adaptin beta aa. 75-245
    Klon
    74-Adaptin beta
    Isotyp
    IgG1
    Top Product
    Discover our top product AP2B1 Primärantikörper
  • Applikationshinweise
    Bioimaging
    1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
    2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
    3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
    4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
    5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
    6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
    7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
    8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
    9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
    10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    11. View and analyze the cells on an appropriate imaging instrument.
    Kommentare

    Related Products: ABIN968537, ABIN967389

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store undiluted at -20°C.
  • Naga Prasad, Laporte, Chamberlain, Caron, Barak, Rockman: "Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex." in: The Journal of cell biology, Vol. 158, Issue 3, pp. 563-75, (2002) (PubMed).

    Ros-Baro, Lopez-Iglesias, Peiro, Bellido, Palacin, Zorzano, Camps: "Lipid rafts are required for GLUT4 internalization in adipose cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 21, pp. 12050-5, (2001) (PubMed).

    Laporte, Oakley, Zhang, Holt, Ferguson, Caron, Barak: "The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3712-7, (1999) (PubMed).

    Ponnambalam, Robinson, Jackson, Peiperl, Parham: "Conservation and diversity in families of coated vesicle adaptins." in: The Journal of biological chemistry, Vol. 265, Issue 9, pp. 4814-20, (1990) (PubMed).

  • Target
    AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))
    Andere Bezeichnung
    Adaptin beta (AP2B1 Produkte)
    Synonyme
    5-HT(2B) antikoerper, 5-HT2B antikoerper, ADTB2 antikoerper, AP105B antikoerper, AP2-BETA antikoerper, CLAPB1 antikoerper, 1300012O03Rik antikoerper, AI788979 antikoerper, fa16e07 antikoerper, wu:fa16c06 antikoerper, wu:fa16e07 antikoerper, wu:fc18c08 antikoerper, zgc:55659 antikoerper, 5-hydroxytryptamine receptor 2B antikoerper, adaptor related protein complex 2 beta 1 subunit antikoerper, adaptor-related protein complex 2, beta 1 subunit antikoerper, adaptor related protein complex 2 beta 1 subunit L homeolog antikoerper, HTR2B antikoerper, AP2B1 antikoerper, Ap2b1 antikoerper, ap2b1 antikoerper, ap2b1.L antikoerper
    Hintergrund
    Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. beta Adaptin subunits (beta1, beta2, beta3, beta4) lack sequence homology to adaptins alpha, gamma, delta, and epsilon, but all of these subunits share a similar domain structure. Adaptin beta1 (also known as Adaptin beta') and beta2 (also known as Adaptin beta) have 83% amino acid identity and are found in the AP1 and AP2 complexes, respectively.
    Molekulargewicht
    106 kDa
    Pathways
    EGFR Signaling Pathway, Neurotrophin Signalübertragung, EGFR Downregulation
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