RAB5 Antikörper (AA 1-215)

Produktnummer ABIN967881

Produktdetails anti-RAB5 Antikörper

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Bindungsspezifität: AA 1-215

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAB5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rab5 aa. 1-215

Klon: 15-Rab5

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RAB5 Antikörper (ABIN967881)

Wang, Hu, Zimmermann, Kilimann: "Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha -helix motifs." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32480-8, (2001) (PubMed).

Pinkoski, Hobman, Heibein, Tomaselli, Li, Seth, Froelich, Bleackley: "Entry and trafficking of granzyme B in target cells during granzyme B-perforin-mediated apoptosis." in: Blood, Vol. 92, Issue 3, pp. 1044-54, (1998) (PubMed).

Li, Stahl: "Structure-function relationship of the small GTPase rab5." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

Details zu RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Andere Bezeichnung: Rab5 (RAB5A Produkte)

Synonyme: RAB5 antikoerper, 2410015H04Rik antikoerper, AI663973 antikoerper, AU021172 antikoerper, nnyRab5a antikoerper, DDBDRAFT_0168594 antikoerper, DDBDRAFT_0229401 antikoerper, DDB_0168594 antikoerper, DDB_0229401 antikoerper, rab5 antikoerper, RAB5A antikoerper, Rab5a antikoerper, rab5al antikoerper, zgc:56644 antikoerper, fj38g08 antikoerper, hm:zehn1144 antikoerper, rab5a antikoerper, wu:fj38g08 antikoerper, RAB5A, member RAS oncogene family antikoerper, rab5A protein antikoerper, Rab5a, GTPase antikoerper, predicted protein antikoerper, Rab GTPase antikoerper, RAB5A, member RAS oncogene family L homeolog antikoerper, RAB5A, member RAS oncogene family, b antikoerper, RAB5A, member RAS oncogene family, a antikoerper, RAB5A antikoerper, Rab5a antikoerper, rab5A antikoerper, rab5a antikoerper, rab5a.L antikoerper, rab5ab antikoerper, rab5aa antikoerper

Hintergrund: Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation, which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 is involved in regulation of early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and organelle membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 insertion into membranes is a multistep process in which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

Molekulargewicht: 25 kDa

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity