EPS8 Antikörper (AA 628-821)

Produktnummer ABIN967774

Produktdetails anti-EPS8 Antikörper

Target: EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8))

Bindungsspezifität: AA 628-821

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPS8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Human, Ratte (Rattus), Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Eps8 aa. 628-821

Klon: 15-Eps8

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968550

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-EPS8 Antikörper (ABIN967774)

Burke, Schooler, Wiley: "Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1897-910, (2001) (PubMed).

Miyamoto, Teramoto, Gutkind, Yamada: "Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors." in: The Journal of cell biology, Vol. 135, Issue 6 Pt 1, pp. 1633-42, (1997) (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, (1992) (PubMed).

Details zu EPS8

Target: EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8))

Andere Bezeichnung: Eps8 (EPS8 Produkte)

Synonyme: AW261790 antikoerper, MGC81285 antikoerper, MGC146320 antikoerper, zgc:56041 antikoerper, epidermal growth factor receptor pathway substrate 8 antikoerper, epidermal growth factor receptor pathway substrate 8 L homeolog antikoerper, EPS8 antikoerper, Eps8 antikoerper, eps8.L antikoerper, eps8 antikoerper

Hintergrund: The p97 [Eps8] protein, a substrate for EGF-R tyrosine kinase, contains an SH3 domain, but lacks a functional SH2 domain. Antibodies to Eps8 recognize the 97 kDa protein and a less abundant 68 kDa protein. Both forms are tyrosine-phosphorylated following treatment of cells with EGF. It is likely that p68 [Eps8] is synthesized from an alternatively spliced mRNA since two major Eps8-specific mRNAs are detected by Northern analysis. Co-immunoprecipitation studies have demonstrated a physical association between the Eps8 protein and the EGF-R both in vivo and in vitro. For many EGF-R substrates, this interaction is mediated through an SH2 domain of the substrate. Since Eps8 lacks a well defined SH2 domain and a fusion protein containing the SH2-like region of Eps8 could not bind EGF-R, the mechanism of Eps8-EGF-R association remains unclear. Overexpression of Eps8 in fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF, suggesting that Eps8 has a role in the modulation of EGF-R function.

Molekulargewicht: 97 kDa

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization