Caveolin-1 Antikörper (AA 1-97)

Produktnummer ABIN967713

Produktdetails anti-Caveolin-1 Antikörper

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Bindungsspezifität: AA 1-97

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caveolin-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)

Kreuzreaktivität: Hund, Maus, Kaninchen, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Caveolin 1 aa. 1-97

Klon: C060

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Kommentare:

Related Products: ABIN967389, ABIN968536

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Caveolin-1 Antikörper (ABIN967713)

Peiró, Comella, Enrich, Martín-Zanca, Rocamora: "PC12 cells have caveolae that contain TrkA. Caveolae-disrupting drugs inhibit nerve growth factor-induced, but not epidermal growth factor-induced, MAPK phosphorylation." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 37846-52, (2000) (PubMed).

de Weerd, Leeb-Lundberg: "Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17858-66, (1997) (PubMed).

Stan, Roberts, Predescu, Ihida, Saucan, Ghitescu, Palade: "Immunoisolation and partial characterization of endothelial plasmalemmal vesicles (caveolae)." in: Molecular biology of the cell, Vol. 8, Issue 4, pp. 595-605, (1997) (PubMed).

Conrad, Smart, Ying, Anderson, Bloom: "Caveolin cycles between plasma membrane caveolae and the Golgi complex by microtubule-dependent and microtubule-independent steps." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 1, pp. 1421-33, (1996) (PubMed).

Li, Okamoto, Chun, Sargiacomo, Casanova, Hansen, Nishimoto, Lisanti: "Evidence for a regulated interaction between heterotrimeric G proteins and caveolin." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15693-701, (1995) (PubMed).

Details zu Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Andere Bezeichnung: Caveolin 1 (CAV1 Produkte)

Synonyme: fc07c04 antikoerper, wu:fc07c04 antikoerper, Cav-1 antikoerper, bscl3 antikoerper, cav1 antikoerper, cav1a antikoerper, cgl3 antikoerper, vip21 antikoerper, cav1b antikoerper, caveolin-1 antikoerper, cav antikoerper, mstp085 antikoerper, Caveolin-1 antikoerper, CAV1 antikoerper, cav-1 antikoerper, Cav antikoerper, caveolin 1 antikoerper, BSCL3 antikoerper, CGL3 antikoerper, MSTP085 antikoerper, PPH3 antikoerper, VIP21 antikoerper, caveolin 1 antikoerper, caveolin 1 L homeolog antikoerper, caveolin 1 S homeolog antikoerper, caveolin 1, caveolae protein antikoerper, caveolin 1, caveolae protein, 22kDa antikoerper, Caveolin antikoerper, cav1 antikoerper, cav1.L antikoerper, cav1.S antikoerper, CAV1 antikoerper, Cav1 antikoerper, cav-1 antikoerper

Hintergrund: Identified as a tyrosine phosphorylated protein in Rous sarcoma virus-transformed chick embryo fibroblasts (CEF), caveolin is now known to be ubiquitously expressed. Caveolin (also known as VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. This transmembrane protein plays a structural role in these specializations. Caveolin is also present at the trans-Golgi network (TGN) and similar quantities are found in apically and basolaterally destined transport vesicles. Caveolin is part of a complex containing glycosylphosphatidylinositol (GPI)-linked molecules and cytoplasmic signaling proteins. Caveolin is a transmembrane adaptor molecule that can simultaneously recognize GPI-linked proteins and interact with downstream cytoplasmic signaling molecules, such as c-yes, Annexin II, and hetero-trimeric G proteins. Caveolin-1 can generate two forms, alpha and ß, due to alternate splicing of the mRNA. Caveolin-1 forms large lipid-binding homo-oligomers which are believed to play a role in caveolae formation. It may also function as a scaffolding protein which concentrates and organizes signaling molecules, a role supported by the fact that caveolin-1 interacts directly with inactive Ras and G-protein alpha subunits.

Molekulargewicht: 22 kDa

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals