RASA1 Antikörper (AA 381-390)

Produktnummer ABIN967706

Produktdetails anti-RASA1 Antikörper

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Bindungsspezifität: AA 381-390

Reaktivität: Human, Ratte, Maus, Hund, Huhn, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RASA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Huhn, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ras-GAP aa.381-390

Klon: 13-RAS

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968619, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RASA1 Antikörper (ABIN967706)

Cichowski, Santiago, Jardim, Johnson, Jacks: "Dynamic regulation of the Ras pathway via proteolysis of the NF1 tumor suppressor." in: Genes & development, Vol. 17, Issue 4, pp. 449-54, (2003) (PubMed).

Sato, Ogata, De Luca: "Annexin V inhibits the 12-O-tetradecanoylphorbol-13-acetate-induced activation of Ras/extracellular signal-regulated kinase (ERK) signaling pathway upstream of Shc in MCF-7 cells." in: Oncogene, Vol. 19, Issue 25, pp. 2904-12, (2000) (PubMed).

Dupont, Blancq: "Formation of complexes involving RasGAP and p190 RhoGAP during morphogenetic events of the gastrulation in xenopus." in: European journal of biochemistry / FEBS, Vol. 265, Issue 2, pp. 530-8, (1999) (PubMed).

Kaplan, Morrison, Wong, McCormick, Williams: "PDGF beta-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex." in: Cell, Vol. 61, Issue 1, pp. 125-33, (1990) (PubMed).

Molloy, Bottaro, Fleming, Marshall, Gibbs, Aaronson: "PDGF induction of tyrosine phosphorylation of GTPase activating protein." in: Nature, Vol. 342, Issue 6250, pp. 711-4, (1990) (PubMed).

Details zu RASA1

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Andere Bezeichnung: Ras-GAP (RASA1 Produkte)

Synonyme: CG9209 antikoerper, D-RasGAP antikoerper, Dmel\\CG9209 antikoerper, RAS-GAP antikoerper, RasGAP antikoerper, RasGap antikoerper, rasGap antikoerper, CM-AVM antikoerper, CMAVM antikoerper, GAP antikoerper, PKWS antikoerper, RASA antikoerper, RASGAP antikoerper, p120GAP antikoerper, p120RASGAP antikoerper, GAPX antikoerper, Rasa antikoerper, Gap antikoerper, vacuolar peduncle antikoerper, RAS p21 protein activator 1 antikoerper, vap antikoerper, RASA1 antikoerper, Rasa1 antikoerper

Hintergrund: Ras-GAP, a protein of approximately 120kDa, is thought to serve as a down-regulator of p21ras by stimulating its otherwise weak intrinsic GTPase activity. Both growth factor receptor and oncogene-encoded tyrosine kinases require functional membrane-associated Ras proteins in order to affect mitogenesis. The manner in which p21ras acts as a signal transducer is still a matter of speculation. The Ras-GTPase stimulating activity has been found to reside in the carboxy-terminal region of Ras-GAP, while the amino-terminal region contains two SH2 domains and an intervening SH3 domain. In cells stimulated with EGF or transformed by pp60 [v-src], Ras-GAP becomes phosphorylated on both tyrosine and serine residues and forms distinct complexes with two phosphorylated proteins of 62 and 190kDa. The tyrosine phosphorylation of Ras-GAP may modulate its subcellular localization and activity as a negative regulator of p21ras.
This antibody routinely tested by western blot analysis.

Molekulargewicht: 120 kDa

Pathways: Regulation of Actin Filament Polymerization, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals