TNFRSF1A Antikörper

Produktnummer ABIN967653

Produktdetails anti-TNFRSF1A Antikörper

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser TNFRSF1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse TNFRI

Klon: 55R-286

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: 1) ELISA Capture:
The purified 55R-286 antibody (ABIN967653) is useful as a capture antibody for a sandwich ELISA for measuring mouse TNFRI protein levels. Purified 55R-286 antibody can be paired with the biotinylated 55R-170 hamster anti-mouse TNFRI as the detecting antibody. Purified 55R-286 antibody should be titrated 4 - 8 µg/ml to determine its optimal concentration for ELISA capture.
Note: This ELISA antibody pair shows no cross-reactivity with other purified recombinant cytokines or receptors that were tested including:
1) Mouse IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12 p40, IL-12p70, IL-16, IL-17, GM-CSF, IFN-gamma, TNF, TNFRI, TNFRII, MCP-1, CRG-2,
2) Human IL-1alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-16, GM-CSF, G-CSF, TNF, LT-alpha, TNFRI, TNFRII, MCP-1, MCP-2, IFN-gamma, RANTES, MIG
3) Rat IL-1alpha, IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, MCP-1, TNF

2) Immunofluorescent Staining and Flow Cytometric Analysis:
The purified form of 55R-286 (ABIN967653) can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10^6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of TNFRI. An appropriate purified isotype control is clone A19-3. A three-layer staining protocol is recommended for maximizing the detection of TNFRI expressed by cells as detailed in the figure legend.
Note: 55R-286 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where ligands (eg, TNF) for TNF receptors are present. Based on our testing results (data not shown), the presence of exogenous recombinant mouse TNF at levels Less or equal than 1 µg per 10^6 cells was insufficient to inhibit the binding of 55R-286 to L929 cells that express p55 TNFR (at 0.25 µg antibody/10^6 cells). Please note also that as a consequence of in vivo or in vitro activation, cell surface TNFRI can either be shed by cells or transiently expressed at higher levels. As a result, cellular activation can affect the cells overall expressed level of surface TNFRI.

Immunoprecipitation:
The 55R-286 antibody has been reported to be useful for the immunoprecipitation of p55 TNFR from lysates of mouse Meth A fibrosarcoma cells. Please note that this application is not routinely tested.

WB:
The purified 55R-286 antibody has been found useful for Western blotting. Please note that this application is not routinely tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-TNFRSF1A Antikörper (ABIN967653)

Pinckard, Sheehan, Schreiber: "Ligand-induced formation of p55 and p75 tumor necrosis factor receptor heterocomplexes on intact cells." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10784-9, (1997) (PubMed).

Pinckard, Sheehan, Arthur, Schreiber: "Constitutive shedding of both p55 and p75 murine TNF receptors in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 8, pp. 3869-73, (1997) (PubMed).

Sheehan, Pinckard, Arthur, Dehner, Goeddel, Schreiber: "Monoclonal antibodies specific for murine p55 and p75 tumor necrosis factor receptors: identification of a novel in vivo role for p75." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 607-17, (1995) (PubMed).

Details zu TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Andere Bezeichnung: CD120a (TNFRSF1A Produkte)

Synonyme: CD120a antikoerper, FPF antikoerper, MS5 antikoerper, TBP1 antikoerper, TNF-R antikoerper, TNF-R-I antikoerper, TNF-R55 antikoerper, TNFAR antikoerper, TNFR1 antikoerper, TNFR1-d2 antikoerper, TNFR55 antikoerper, TNFR60 antikoerper, p55 antikoerper, p55-R antikoerper, p60 antikoerper, TNF-R1 antikoerper, TNF-alphaR1 antikoerper, TNFRI antikoerper, TNFRp55 antikoerper, TNFalpha-R1 antikoerper, Tnfr-2 antikoerper, Tnfr1 antikoerper, fpf antikoerper, tbp1 antikoerper, p55-r antikoerper, tnf-r antikoerper, tnfar antikoerper, tnfr1 antikoerper, cd120a antikoerper, tnfr55 antikoerper, tnfr60 antikoerper, xtnfr1 antikoerper, wu:fj65b10 antikoerper, zgc:85914 antikoerper, TNFR I antikoerper, TNFRSF1A antikoerper, TNFA antikoerper, cTNF antikoerper, TNF receptor superfamily member 1A antikoerper, tumor necrosis factor receptor superfamily, member 1a antikoerper, TNF receptor superfamily member 1A S homeolog antikoerper, tumor necrosis factor antikoerper, TNFRSF1A antikoerper, Tnfrsf1a antikoerper, tnfrsf1a.S antikoerper, tnfrsf1a antikoerper, TNF antikoerper

Hintergrund: The 55R-286 antibody reacts with the extracellular region of the 55 kDa receptor for the mouse cytokines, Tumor Necrosis Factor (TNF) and lymphotoxin-alpha (LT-alpha). This receptor, referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI) [aka, CD120a], is expressed by a variety of cell lines and normal cell types including T cells, monocytes, macrophages, and neutrophils. B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes are uniformly negative for TNFRI expression. In addition, the 55R-286 antibody can bind to a soluble, truncated form of the mouse Type I TNFR that is shed by cells in response to certain stimuli, e.g., cells treated with LPS or TNF. The in vivo administration of nonblocking, nonagonistic 55R-286 antibody reportedly results in the linear accumulation of shed, soluble forms of the TNFRI in the circulation. The 55R-286 antibody does not recognize the 75 kD (p75) Type II TNFR (aka, CD120b). 55R-286 does not block the binding and does not neutralize the bioactivity of the TNF ligand on L929 target cell populations that express TNFRI. The immunogen used to generate the 55R-286 hybridoma was a purified, soluble extracellular domain of the mouse TNFRI.
Synonyms: TNF Receptor, Type I/p55

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway