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CD44 Antikörper

CD44 Reaktivität: Maus FACS, WB, ELISA, IHC (fro), IHC (f), BR Wirt: Ratte Monoclonal KM114 unconjugated
Produktnummer ABIN967622
  • Target Alle CD44 Antikörper anzeigen
    CD44
    Reaktivität
    • 310
    • 169
    • 81
    • 30
    • 25
    • 24
    • 16
    • 11
    • 9
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 172
    • 123
    • 94
    • 2
    • 1
    Ratte
    Klonalität
    • 274
    • 116
    • 2
    Monoklonal
    Konjugat
    • 171
    • 44
    • 31
    • 27
    • 19
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD44 Antikörper ist unkonjugiert
    Applikation
    • 274
    • 156
    • 94
    • 76
    • 72
    • 72
    • 62
    • 40
    • 37
    • 31
    • 30
    • 13
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Reagent (BR)
    Marke
    BD Pharmingen™
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell clone BMS2
    Klon
    KM114
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product CD44 Primärantikörper
  • Applikationshinweise
    For quantitation of soluble CD44, a sandwich ELISA using purified IM7 antibody for capture and biotinylated KM114 mAb for detection has been described. For IHC, we recommend the use of purified IM7 mAb in our special formulation for immunohistochemistry.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4° C.
  • Castro, Bono, Simon, Vargas, Rosemblatt: "Spleen-derived stromal cells. Adhesion molecules expression and lymphocyte adhesion to reticular cells." in: European journal of cell biology, Vol. 74, Issue 4, pp. 321-8, (1998) (PubMed).

    Naor, Sionov, Ish-Shalom: "CD44: structure, function, and association with the malignant process." in: Advances in cancer research, Vol. 71, Issue 4, pp. 241-319, (1997) (PubMed).

    Weber, Ashkar, Glimcher, Cantor: "Receptor-ligand interaction between CD44 and osteopontin (Eta-1)." in: Science (New York, N.Y.), Vol. 271, Issue 5248, pp. 509-12, (1996) (PubMed).

    Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

    Katoh, Zheng, Oritani, Shimozato, Kincade: "Glycosylation of CD44 negatively regulates its recognition of hyaluronan." in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 419-29, (1995) (PubMed).

    Zheng, Katoh, He, Oritani, Miyake, Lesley, Hyman, Hamik, Parkhouse, Farr, Kincade: "Monoclonal antibodies to CD44 and their influence on hyaluronan recognition." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 485-95, (1995) (PubMed).

    Katoh, McCarthy, Kincade: "Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3440-9, (1994) (PubMed).

    Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, (1994) (PubMed).

    Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

    Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, (1993) (PubMed).

    Murakami, Miyake, June, Kincade, Hodes: "IL-5 induces a Pgp-1 (CD44) bright B cell subpopulation that is highly enriched in proliferative and Ig secretory activity and binds to hyaluronate." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3618-27, (1991) (PubMed).

    MacDonald, Budd, Cerottini: "Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes." in: Current topics in microbiology and immunology, Vol. 159, Issue 4, pp. 97-109, (1990) (PubMed).

    Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, (1990) (PubMed).

    Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." in: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, (1989) (PubMed).

  • Target
    CD44
    Andere Bezeichnung
    CD44 (Pgp-1, H-CAM, Ly24) (CD44 Produkte)
    Synonyme
    CDW44 antikoerper, CSPG8 antikoerper, ECMR-III antikoerper, HCELL antikoerper, HUTCH-I antikoerper, IN antikoerper, LHR antikoerper, MC56 antikoerper, MDU2 antikoerper, MDU3 antikoerper, MIC4 antikoerper, Pgp1 antikoerper, AU023126 antikoerper, AW121933 antikoerper, AW146109 antikoerper, HERMES antikoerper, Ly-24 antikoerper, Pgp-1 antikoerper, CD44A antikoerper, METAA antikoerper, RHAMM antikoerper, XCD44 antikoerper, cd44 antikoerper, cdw44 antikoerper, cspg8 antikoerper, ecmr-iii antikoerper, hcell antikoerper, lhr antikoerper, mc56 antikoerper, mdu2 antikoerper, mdu3 antikoerper, mic4 antikoerper, mutch-i antikoerper, pgp1 antikoerper, CD44 molecule (Indian blood group) antikoerper, syndecan 4 antikoerper, CD44 antigen antikoerper, CD44 molecule (Indian blood group) S homeolog antikoerper, CD44 antikoerper, SDC4 antikoerper, Cd44 antikoerper, cd44.S antikoerper
    Hintergrund
    The KM114 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The KM114 hybridoma was produced at the same time as the published clone KM201, and the mAb recognizes a different epitope from that recognized by mAb IM7. The standard form of CD44, lacking variable exons and referred to as CD44H or CD44S, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have lower expression of CD44H on T cells. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1).10 Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells, and memory cells can be recognized by their CD44[hi] phenotype. KM114 antibody can be used in ELISA to detect soluble CD44, and it is effective for in vitro blocking of hyaluronate recognition by CD44. It has been reported that KM114 mAb cross-reacts with CD44 of the Chinese hamster, Cricetulus griseus, but not human CD44.
    Synonyms: Pgp-1, H-CAM, Ly-24
    Pathways
    Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling
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