CREB and ATF-1 (pSer133) Antikörper

Produktnummer ABIN967591

Produktdetails

Target: CREB and ATF-1

Bindungsspezifität: pSer133

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Western Blotting (WB)

Marke: BD Pharmingen™

Kreuzreaktivität (Details): Predicted: Mouse, Rat

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human CREB Peptide

Klon: J151-21

Isotyp: IgG1 kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Shaywitz, Greenberg: "CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals." in: Annual review of biochemistry, Vol. 68, Issue 2, pp. 821-61, (2000) (PubMed).

Antigendetails

Target: CREB and ATF-1

Hintergrund: Transcription of various genes is regulated by the cyclic AMP (cAMP) signal transduction pathway through a family of cAMP Responsive Element (CRE)-Binding transcription factors that include CREB, CREM (CRE Modulator), and ATF-1 (Activating Transcription Factor 1). The genes for these transcription factors encode multiple isoforms that are created by alternative splicing, alternative initiation codons, and alternative intronic promoters. Homo- and heterodimers of these members of the basic domain-and-leucine zipper superfamily of proteins bind the palindromic TGACGTCA sequence of CRE. CREB is expressed in all somatic cells, and many different stimuli activate CREB by phosphorylation of its serine 133 (S133). The transcriptional activity and specificity of CREB are regulated by the signaling pathways initiated by the various stimuli. Transcriptional regulation by CREB family members has been implicated in many physiological responses to extracellular and environmental stimuli, such as memory, tissue growth and development, and homeostasis.
The J151-21 monoclonal antibody recognizes the phosphorylated S133 of human CREB in addition to cross-reacting with S63 of human ATF-1.

Molekulargewicht: 43 kDa